 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Combination of Laccase, Xylanase and Cellulase in Lignocellulose
Degradation by White Rot Fungi, Lentinus polychrous Lev. and
L. squarrosulus Mont.) ผู้เขียน: Charida Pukahuta,  นางพูนพิไล สุวรรณฤทธิ์, รองศาสตราจารย์ , Emiko Shianagawa, Hisashi Hoshida, Yoshinori Nishizawa สื่อสิ่งพิมพ์:pdf AbstractThe 22 isolates of white rot fungi, Lentinus spp. were screened for their activity of cellulase,
xylanase and laccase. L. polychrous Lev. LP-PT-1 was cultured in 8 liquid media and 2 solid media. These
10 media were tested for lignocellulose degradation. Potato dextrose broth (PDB) and Fahraeus broth
with 2 % rice straw (FRS) were selected as appropriate media. Both media were conducted to cultivate
4 isolates of L. polychrous Lev. LP-PT-1, LP-SW-3, LP-WR-13 and LP-UD-1 and 1 isolate of L.
squarrosulus Mont.LS-YA. The xylanase and cellulase activities were determined at 40?C. Xylidine
induced higher laccase actvity in 4 isolates, with the exception of LP-PT-1. LP-PT-1 produced three
enzyme actvities in both PDB and FRS with and without xylidine addition. LP-SW-3 produced the highest
activity of xylanase and laccase, 13.1 mU/ml and 3.27 U/ml, respectively, in FRS with xylidine addition.
LP-SW-3, LP-WR-13 and LP-UD-1 showed the activity of three enzymes in FRS but lacked cellulase
and/or xylanase activities in PDB. The production of xylanase of LP-SW-3 in FRS was induced by rice
straw and of LP-UD-1 in PDB was induced by xylidine. LS-YA had the activity of all three enzymes in
PDB but lacked xylanase activity in FRS without xylidine addition. FRS and xylidine addition was used
to produce the culture broth for bleaching pulp. The increase in brightness of the treated pulp, 1.5-2.0 %
higher than the control pulp, was correlated with the detected activity of xylanase of L. polychrous
Lev.LP-PT-1, LP-UD-1 and L. squarrosulus Mont.LS-YA with low activity of laccase in each isolate.
The high and moderate activity of xylanase with high and moderate activity of laccase in L. polychrous
Lev. LP-SW-3 and LP-WR-13 did not increase the brightness of the treated pulp. It was concluded that
these thermotolerant white rot fungi have the different combination of enzyme systems on lignocellulose
degradation. The bleaching of pulp beneficially effected by the xylanase activity only when the activity
of laccase was low. |