 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Evaluation of Membrane Fragments Extracted from Escherichia coli and Pseudomonas aeruginosa on Campylobacter jejuni Growth under Normal Atmosphere) ผู้เขียน: Pattara Wongjaroen, Kanokwan Kittiniyom, Potjanee Srimanote, Wacharee Tiyasuttipan, Wijit Wonglumsom สื่อสิ่งพิมพ์:pdf AbstractStudy on extraction of membrane fragments from Escherichia coli and Psuedomonas aeruginosa were determined for using to support Campylobacter cultivation under normal atmosphere. Crude membrane fragments were extracted from 45 strains of E. coli and 44 strains of P. aeruginosa. Strains that provided highest efficiency of oxygen reduction were selected to purify membrane fragments by French pressure cell and ultracentrifugation. The purified membrane fragments were characterized and investigated for supporting Campylobacter jejuni growth in Mueller-Hinton broth. The broth supplemented with and without membrane fragments was incubated under normal atmosphere at 37?C or 42?C. Campylobacter growth in the broth containing purified membrane fragments was initially observed within 6 to 12 hours of incubation while the media without supplemented with membrane fragments showed no growth of the bacteria. Therefore, oxygen reducing membrane fragments prepared from the selected strains could support Campylobacter cultivation under normal atmosphere. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : External Quality Assessment in Isolationand Identification of Bacteria) ผู้เขียน:Wijit Wonglumsom, Chaniya Leepiyasakulchai, Wacharee Tiyasuttipan สื่อสิ่งพิมพ์:pdf AbstractExternal quality assessment for evaluation of individual competency in isolation and identification of bacteria for clinical microbiological laboratory was studied. Lyophilized or freezedried bacteria were prepared and a panel of lyophilized bacteria was shipped once a year. The first set consisted of two mixed cultures and one pure culture. Forty-three laboratories participated in this first set. The second set provided three ampoules of lyophilized mixed bacteria. Thirty-eight laboratories participated in this second set. Fifteen (34.9%) laboratories and 21 (55.3%) laboratories were correctly reported for the first and second proficiency sets, respectively. Of 33 laboratories participated in both trials, fifteen (46%) laboratories showed performance improvement. A meeting concerning on quality assurance and good laboratory practices in clinical microbiology was also provided for participating laboratories before shipment of the second panel. This pilot scheme showed a high possibility of establishing the External quality assessment scheme in bacterial isolation and identification for continuous improvement of quality assurance. |