 หัวเรื่อง:การเพาะเลี้ยงเนื้อเยื่อถั่วแดง ผู้เขียน: นางสิรนุช ลามศรีจันทร์, ศาสตราจารย์ , นางอรุณี วงศ์ปิยะสถิตย์, ศาสตราจารย์,  Varaporn Rojanasiriwong สื่อสิ่งพิมพ์:pdf AbstractA procedure for regenerating plantlets through organogenesis from tissue culture of red kidney bean was attempted. Seeds of Mecosta cultivar were germinated on MS supplemented with 11.0 mg/l BA. Cotyledonary nodes were excised from 14 day-old-seedling and then cultured on MS to which 0.05, 0.5 and 1 mg/l IAA and NAA in combination with 1.0 and 5 mg/l kinetin and 1.0, 5.0 and 11 mg/l BA were added. Multiple shoot buds were produced only on MS + 0.5 mg/l IAA +11 mg/l BA; MS + 0.5 mg/l NAA + 5 mg/l BA and MS + 1 mg/l NAA +11 mg/l BA. For further shoot development and elongation, shoot buds were cultured on MS, L6 and RB1 containing 1.0 mg/l BA. The highest number of developed shoots were observed on MS while the RB1 yielded the poorest shoot development. |