ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Development of a Quantitative, Competitive-PCR (QC-PCR) Assay to Determine the DNA Load of Porcine Circovirus Type 2 (PCV2) in Blood and Fecal Swabs)

ผู้เขียน:Nattarat Thangthumniyom, Tippawan Juntafong, Nuntawan Petcharat, ดร.ปริวรรต พูลเพิ่ม, ผู้ช่วยศาสตราจารย์, ดร.เฉลิมพล เล็กเจริญสุข, ผู้ช่วยศาสตราจารย์, ดร.พรทิพภา เล็กเจริญสุข, ศาสตราจารย์

สื่อสิ่งพิมพ์:pdf

Abstract

PCV2 is an essential causative agent of post-weaning multisystemic wasting syndrome (PMWS). The detection of PCV2 is not the defi nitive diagnosis since PCV2 infection does not always lead to PMWS; however, viral load may relate to PCV2-induced PMWS. In this study, a quantitative, competitive-polymerase chain reaction (QC-PCR) assay was developed to determine the amount of PCV DNA in whole blood and fecal swabs of pigs from PMWS-affected and PMWS-nonaffected farms. The QC-PCR was based on competitive co-amplifi cation of a 345 bp fragment of the PCV 2 in the samples with a known concentration of the competitor DNA, which produced a 513 bp fragment. Blood and fecal swabs were collected from 140 pigs from 11 PMWS-affected and 14 PMWS-nonaffected farms. The results demonstrated that the PCV2 DNA from fecal swabs of pigs in the PMWS-affected farms ranged from less than 1 fg.?L-1 to 100 pg.?L-1 with a mean PCV2 DNA concentration of 6.42 ? 107 copies.mL-1, which was signifi cantly higher than that from PMWS-nonaffected farms (3.8 ? 105 copies. mL-1). The results indicate correlation of PCV2 viremia and shedding to the development of PMWS. Therefore, the QC-PCR technique developed here could be applied as a tool to predict trends of the emergence and spread of the disease.

Article Info

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 045, Issue 6, Nov 11 - Dec 11, Page 1028 - 1037 |  PDF |  Page