 ผลงานตีพิมพ์ในวารสารวิชาการProcessing and secretion of non-cognate bacteriocins by EnkT, an ABC transporter from a multiple-bacteriocin producer, Enterococcus faecium NKR-5-3ผู้แต่ง: Hirotoshi Sushida, Miyuki Sakei, Rodney H.Perez, Naoki Ishibashi, Takeshi Zendo,  Dr.Pongtep Wilaipun, Assistant Professor , Dr.Vichien Leelawatcharamas, Associate Professor , Jiro Nakayama, Kenji Sonomoto, วารสาร: 
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ผลงานตีพิมพ์ในวารสารวิชาการMolecular characterization of the possible regulation of multiple bacteriocin production through a three-component regulatory system in Enterococcus faeciumNKR-5-3ผู้แต่ง:Naoki Ishibashi, Naho Matsumoto, Rodney Honrada Perez, Shun Iwatani, Haruki Sugino, Takeshi Zendo, Dr.Pongtep Wilaipun, Assistant Professor, Dr.Vichien Leelawatcharamas, Associate Professor, Jiro Nakayama, Kenji Sonomoto, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการInfluence of Physical Factors and Various Complex Media on Growth and Bacteriocin Production of Two-synergistic Peptide with Heat Stable Bacteriocin Producer, Enterococcus faecium NKR-5-3, Isolated from Thai Fermented Fishผู้แต่ง:Dr.Pongtep Wilaipun, Assistant Professor, Takeshi Zendo, Mrs.Mathana Sangjindavong, Associate Professor , Dr.Sunee Nitisinprasert, Associate Professor , Dr.Vichien Leelawatcharamas, Associate Professor, Jiro Nakayama, Kenji Sonomoto, วารสาร: |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Screening and Characterization of Bacteriocin Producing Lactic Acid Bacteria Isolated from Chicken Intestine) ผู้เขียน:คมแข พิลาสมบัติ, วรวิทย์ วัชชวัลคุ, ดร.สุนีย์ นิธิสินประเสริฐ, รองศาสตราจารย์, Adisorn Swetwiwathana, Takeshi Zendo, Jiro Nakayama, Kenji Sonomoto, ดร.ธวัชชัย ศักดิ์ภู่อร่าม, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractFive hundred strains of lactic acid bacteria (LAB) were isolated from chicken intestine and screened for their abilities to produce bacteriocins. One strain, K4, produced a bacteriocin with activity against Lactobacillus sakei subsp. sakei JCM 1157T, Leuconostoc mesenteroides subsp. mesenteroides JCM 6124T, Bacillus coagulans JCM 2257T, Enterococcus feacalis JCM 5803T, Listeria innocua ATCC 33090T and Brochotrix campestris NBRC 11547T. The antibacterial activity was inactivated by proteolytic enzymes, indicating that it has proteinaceous structure of a bacteriocin. Its properties were heat-tolerance at 121?C for 15 min and stable at wide pH range of 3-10. The mode of action of this bacteriocin was clearly bactericidal activity against Lb. sakei subsp. sakei JCM 1157T and B. coagulans JCM 2257T whereas it showed bacteriostatic action against Br. campestris NBRC 11547T. In addition, K4 strain was able to grow and produce bacteriocin in MRS containing 4% NaCl and at wide pH range of 3-9. Based on morphological, biochemical characteristics and 16S rDNA nucleotide sequence analysis, K4 strain was identified as Lactobacillus salivarius. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Influence of Physical Factors and Various Complex Media on Growth and Bacteriocin Production of Two-synergistic Peptide with Heat Stable Bacteriocin Producer, Enterococcus f ผู้เขียน:ดร.พงษ์เทพ วิไลพันธ์, ผู้ช่วยศาสตราจารย์, Takeshi Zendo, นางมัทนา แสงจินดาวงษ์, รองศาสตราจารย์, ดร.สุนีย์ นิธิสินประเสริฐ, รองศาสตราจารย์, ดร.วิเชียร ลีลาวัชรมาศ, รองศาสตราจารย์, Jiro Nakayama, Kenji Sonomoto สื่อสิ่งพิมพ์:pdf AbstractEnterococcus faecium NKR-5-3, isolated from Thai fermented fish (Pla-ra), produced an interested two-synergistic peptide bacteriocin with heat stable and broad spectrum activity. The influence of complex media and culture condition on growth as well as bacteriocin production of E. faecium NKR-5-3 has been studied. From 8 indicator strains used, Enterococcus faecalis ATCC 19433 was found to be the most sensitive strain. The bacteriocin to be diluted with distilled water containing 0.1% (v/v) Tween 80 increased 2-fold to 4-fold of its activity against indicator strains when compared with distilled water. In M-MRS broth, bacteriocin production reached maximum level at 30?C and decreased with the increasing of culture temperature. Among different 6 complex media for lactic acid bacteria cultured at 30?C, M 17 broth with the initial pH of 7.5 yielded the maximum growth and bacteriocin production of E. faecium NKR-5-3. Growth and bacteriocin production decreased when it was cultured in M 17 broth containing more than 1% NaCl and they were completely inhibited in M 17 broth containing more than 9% NaCl. Bacteriocin activity reached a detectable level at the early exponential phase and increased due to the cell growth to the maximum level at the end of exponential phase in 22 hr of incubation, which displayed a primary metabolite production. |
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