Search Result of "Sriwong, C."

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Characterization of the Antibacterial Activity and Probiotic Properties of Lactic Acid Bacteria Isolated from Raw Fish and Nham-Plaa)

ผู้เขียน:ImgSuree Nanasombat, ImgSaranya Phunpruch, ImgNiracha Sriwong, ImgThitirut Jaichalad, ImgWaragul Onnom, ImgSupalak Odthon



Isolates of lactic acid bacteria from fish fillets and nham-plaa were screened to select the most suitable strains for the formulation of starter cultures with probiotic properties. Using an agar spot test, 30 of the 180 isolates that were tested inhibited at least one of four indicator strains; Staphylococcus aureus TISTR 118, Pediococcus acidilactici TISTR 051, Lactobacillus bulgaricus TISTR 415 and Listeria monocytogenes DMST 11256. Two bacterial isolates,13IS3 and 13IS4, from fish fillets were resistant to a low pH in lactic acid (pH 2.9-3.2) and in HCl (pH 2.2) and a high concentration of NaCl (6.0%) and bile salts (5.25%). These isolates were identified by morphological, biochemical and molecular analysis as Lactococcus lactis 13IS3 and Lactobacillus sakei 13IS4. The fermentation of nham-plaa model broth (NMB) by L. lactis 13IS3 seemed to be stimulated by 1.0% garlic extract and 0-1.5% NaCl at all temperatures of incubation. L. sakei 13IS4 fermented at a slower rate than L. lactis 13IS3, but fermented more rapidly in a 3.0%-garlic extract NMB with 0-1.5% NaCl at 30?C and in the same medium containing 1.0-1.5% NaCl at 35?C.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 042, Issue 4, Oct 08 - Dec 08, Page 747 - 757 |  PDF |  Page 



ดร. ประกิต สุขใย, รองศาสตราจารย์

ที่ทำงาน:ภาควิชาเทคโนโลยีชีวภาพ คณะอุตสาหกรรมเกษตร

สาขาที่สนใจ:Biodegradability of bioplastics, Nanocellulose, composting