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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Enzymatic Production of Fructo-Oligosaccharides from Sucrose)

ผู้เขียน:Imgดร.สาโรจน์ ศิริศันสนียกุล, ศาสตราจารย์, ImgSittiwat Lertsiri, ImgPreecha Tonsagunrathanachai, ImgPairojana Luangpituksa



To increase an efficiency of fructo-oligosaccharide production from sucrose, a mixed-enzyme system of ?-fructofuranosidase (Aspergillus niger ATCC 20611) and glucose oxidase (Sigma Chemical Co., Ltd.) was carried out in a 2-l stirred tank reactor with the following controlled conditions; temperature 40?C, pH 5.5, aeration rate 1 vvm and agitation rate 550 rpm, for 32 hours of incubation time. With an initial sucrose concentration of 400 g/l, the optimal condition was obtained by using ?-fructofuranosidase and glucose oxidase 10 and 15 units per gram sucrose, respectively. As a result, the yields of kestose (YGF2/S) and nystose (YGF3/S) from sucrose and their volumetric productivities (QGF2/S and QGF3/S) were 0.44, 0.49 g/g, and 4.97, 5.44 g/l h, respectively.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 034, Issue 2, Apr 00 - Jun 00, Page 262 - 269 |  PDF |  Page