Search Result of "Siriphorn Jangsutthivorawat"

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Nucleotide Polymorphism of Starch Synthesis Genes in Thai Rice)

ผู้เขียน:ImgSiriphorn Jangsutthivorawat, ImgHugo Volkaert

สื่อสิ่งพิมพ์:pdf

Abstract

Starch composition and cooking quality are important rice-grain characteristics of interest to breeders. One hundred and ninety-two accessions of Oryza, mostly from Thailand, were assayed using PCR-SSCP and alleles were identified at seven starch-synthesis gene loci (GBSSI, SSSIIIa, SSSIIIb, SSSIVa, SSSIVb, RBE1 and RBE3). The nucleotide sequences were obtained corresponding to each of the SSCP patterns observed and the sequences were submitted to GenBank (accession numbers EF990806 – EF990893). The frequency of nucleotide polymorphisms at these loci was about 1 SNP per 29 bp. A total of 136 nucleotide polymorphisms (SNPs), 16 insertions/deletions (indels) and 4 SSRs were identified. The SSRs were found at the GBSSI (CT, AATT), SSSIVb (GGGTT) and RBE1 (CT) loci. The nucleotide diversity, as measured by ?, ranged from 0.00086 (RBE3) to 0.02747 (SSSIVa) and nucleotide polymorphism, as measured by q, ranged from 0.00154 (RBE3) to 0.02337 (SSSIVa). The Oryza accessions from Thailand in this study displayed a high nucleotide diversity compared with previous estimates of Oryza species based on estimates of multiple loci.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 043, Issue 1, Jan 09 - Mar 09, Page 154 - 163 |  PDF |  Page