 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : A Proteomic Approach to Analyze Rice Bran and Shoots of Kao Dawk Mali 105 and its Mutants) ผู้เขียน: Arunee Trisiriroj, Shui-Tein Chen,  นางนฤมล จียโชค สื่อสิ่งพิมพ์:pdf AbstractProteomic analysis was used to investigate bran proteins from wild-type Oryza sativa L. variety KDML105 and its mutant RD15. Fractionation of bran proteins by sequential solubilization showed about 1,000 total spots in 2-D gel. The 2-D gels revealed the similarity in protein patterns between KDML105 and RD15 because most expressed proteins in bran were house-keeping proteins. Consequently, rice shoots were investigated using etiolated shoots and green shoots of KDML105 and its two mutants, namely RD15 and RD6. Rice shoot proteins were extracted by 10% TCA in acetone. 2-D gels presented a greater number of etiolated proteins than green shoot proteins in all 3 varieties. Due to low expression of RuBisCO enzyme in etiolate, some proteins appeared in higher intensities in etiolated samples than in green shoot samples. As a consequence the proteins were excised for MS analysis. However, most of the different proteins among these three varieties appeared in low intensities. The result of this study revealed that KDML105, RD15 and RD6 expressed different types of salt-stress induced proteins. |