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 ผลงานตีพิมพ์ในวารสารวิชาการSuccessful artificial insemination in the Asian elephant (Elephas maximus) using chilled and frozen-thawed semenผู้แต่ง: Dr.Nikorn Thongthip, Associate Professor ,  Mahasawangkul, S, Thitaram, C, Pongsopavijitr, P, Mr.Kornchai (Mangkorn) Kornkaewrat (Damyang), Dr.Anuchai Pinyopummin, Associate Professor, Angkawanish, T, Jansittiwate, S, Rungsri, R, Boonprasert, K, Wongkalasin, W, Homkong, P, Dejchaisri, S, Dr.Worawidh Wajjwalku, Associate Professor , Saikhun, K, วารสาร:  
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ผลงานตีพิมพ์ในวารสารวิชาการNuclear maturation and development of IVM/IVF canine embryos in synthetic oviductal fluid or in co-culture with buffalo rat liver cellsผู้แต่ง:Saikhun, J, Sriussadaporn, S, Dr.Nikorn Thongthip, Associate Professor, Dr.Anuchai Pinyopummin, Associate Professor, Kitiyanant, Y, วารสาร:  |
ผลงานตีพิมพ์ในวารสารวิชาการRelationship between seminal and serum calcium concentration with semen quality in the Asian elephant (Elephas maximus)ผู้แต่ง:Sivilaikul, S., Jitprom, A., Kularb, A., Kornkaewrut, K., Suthanmaphinuth, P., Mahasawangkul, S., Saikhun, K., Wajjwalku, W., Dr.Nikorn Thongthip, Associate Professor, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการEffect of pre-freeze semen quality, extender and cryoprotectant on the post-thaw quality of Asian elephant (Elephas maximus indicus) semenผู้แต่ง:P. Imrat, Ms.Piyawan Suthunmapinanta , K. Saikhun, S. Mahasawangkul, E. Sostaric, P. Sombutputorn, S. Jansittiwate, Dr.Nikorn Thongthip, Associate Professor, Dr.Anuchai Pinyopummin, Associate Professor, B. Colenbrander, W.V. Holt, T.A.E. Stout, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการSemen characteristics and sperm morphology of serow (Capricornis sumatraensis)ผู้แต่ง:Suwanpugdee, A., Kornkeawrat, K., Saikhun, K., Siriaroonrat, B., Tipkantha, W., Doungsa-ard, K., Sa-ardrit, M., Suthunmapinatha, P., Dr.Anuchai Pinyopummin, Associate Professor, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการUltrastructural alterations of frozen-thawed Asian elephant (Elephas maximus) spermatozoaผู้แต่ง:Sa-Ardrit M., Saikhun J., Dr.Nikorn Thongthip, Associate Professor, Mr.Kornchai (Mangkorn) Kornkaewrat (Damyang), Mahasawangkul S., Angkawanish T., Jansittiwate S., Faisaikarm T., Kitiyanant Y., Pavasuthipaisit K., Dr.Anuchai Pinyopummin, Associate Professor, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการPotential factors affecting semen quality in the Asian elephant (Elephas maximus)ผู้แต่ง:Dr.Nikorn Thongthip, Associate Professor, Saikhun, J, Mahasawangkul, S, Mr.Kornchai (Mangkorn) Kornkaewrat (Damyang), Pongsopavijitr, P, Songsasen, N, Dr.Anuchai Pinyopummin, Associate Professor, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการSeminal Plasma MDA Concentrations Correlating Negatively with Semen Quality in Asian elephantsผู้แต่ง:Dr.Nikorn Thongthip, Associate Professor, Imrat, P, Srihawong, T, Mahasawangkul, S, Dr.Chanin Tirawattanawanich, Assistant Professor, Saikhun, K, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการAssessment of viability and acrosomal status of Asian elephant (Elephas maximus) sperm after treatment with calcium ionophore and heparinผู้แต่ง:Sa-ardrit, M, Dr.Nikorn Thongthip, Associate Professor, Mr.Kornchai (Mangkorn) Kornkaewrat (Damyang), Faisaikarm, T, Kitiyanant, Y, Mahasawangkul, S, Dr.Anuchai Pinyopummin, Associate Professor, Saikhun, K, วารสาร: |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Extender and Storage Time on Motility and Ultrastructure of Cooled-Preserved Boar Spermatozoa) ผู้เขียน:Duangjai Boonkusol, Kulnasan Saikhun, Poonsuk Ratanaphumma สื่อสิ่งพิมพ์:pdf AbstractArtificial insemination (AI) using fresh diluted semen is used worldwide in the porcine industry. Viability of spermatozoa in diluted semen depends on several factors, such as the interaction with the type of extender and storage duration. The aim of this study was to evaluate the effects of extenders and storage time on the motility and ultrastructure of cooled-preserved boar spermatozoa. Semen samples were collected, diluted in BTS, Merck III or Androhep and stored at 15?C for 0, 1, 3, 5 and 7 d. The samples were warmed (37?C) and spermatozoa were evaluated for motility using light microscopy and ultrastructure using scanning (SEM) and transmission (TEM) electron microscopy. The results showed that motility did not differ significantly (P>0.05) among extenders at days 0 to 3, while Androhep showed a significantly (P<0.05) higher percentage of motility than BTS and Merck III from days 5 to 7. At day 7 of semen storage, plasma and acrosomal membrane damage were observed, as revealed by SEM and TEM. These findings suggest that the type of semen extender and cooled-preserved time affected motility and caused structural damage to boar spermatozoa, especially in the plasma and acrosomal membrane. |
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