 หัวเรื่อง:การชักนำให้เกิดการกลายในข้าวโดยวิธีเพาะเลี้ยงเนื้อเยื่อ ตอนที่ 1 ผู้เขียน: P. Pongtongkam, P. Ratisoontorn, S. Suputitada, S. Peyachoknagul, L. Ngernsiri, B. Silayoi, P. Rodrangboon, K. Klakhaeng, R. Senghaphan สื่อสิ่งพิมพ์:pdf AbstractSeeds of Basmati 370 rice variety were cultured on the modified MS medium supplemented with various concentrations of 2,4-D, NAA and kinetin. The suitable callus induction medium was the modified MS medium containign I mg/l 2,4-D, 2 mg/l NAA and 1 mg/l kinetin. Calli from this medium were divided into two groups, one was 3 week old calli (non subcultured calli) and the other was 6 week old calli (subcultured calli). Both groups seemed to grow and develop into plantles quite well on the modified MS medium supplemented with 2 mg/l kinetin. However the percentage of plantles induction from 3 week old calli was better. Calli of both groups were irradiated with different doses of gamma radiation and were cultured on the regenerated medium. The 3 week old calli were better than 6 week old calli in regenerating into plantles. |