Search Result of "S. Apisitiwanich"

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:การเพาะเลี้ยงอับเรณูของข้าวลูกผสม (ขาวดอกมะลิ 105/สไคบอนเนท)

ผู้เขียน:ImgP. Pongtongkam, ImgR. Lervichai, ImgS. Apisitiwanich, ImgS. Peyachoknagul, ImgK. Klakhaeng, ImgP. Sripichit, ImgN. singburaudom, ImgS. Fungfupong, ImgP. Srinives



The anthers of F1 hybrid rice (Khao Dawk Mali 105/Skybonnet) were cultured on the various callus induction media. It was found that a suitable medium for callus induction was N6 containing 2 mg/l 2,4-D, 1 mg/l kinetin, 500 mg/l casein hydrolysate and 50 g/l sucrose. The plantlets could be produced from callus on the MS medium supplemented with 2 mg/l BAP, 1 mg/l kinetin, 0.2 mg/l NAA, 300 mg/l casein hydrolysate, 15% coconut water and 20 g/l sucrose. After transferring these plantlets onto the MS medium containing 1 g/l yeast extract, 15% coconut water, 3 mg/l BAP and 0.01% colchicines for 5 days, about 60% of the doubled haploid plants were recovered.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 029, Issue 2, Apr 95 - Jun 95, Page 158 - 166 |  PDF |  Page