 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Response of Spirulina platensis C1 to High Temperature and High Light Intensity) ผู้เขียน: Ratana Chaiklahan, Nittaya Khonsarn, Nattayaporn Chirasuwan, Marasri Ruengjitchatchawalya, Boosya Bunnag,  นางมรกต ตันติเจริญ สื่อสิ่งพิมพ์:pdf AbstractResponse of Spirulina platensis strain C1 to high temperature and high light intensity was studied. Specific growth rate of the S. platensis C1 cells grown in batch cultures at 35?C with a light intensity of 100 ?E/m2/s was 0.0247 h-1. The specific growth rate decreased to 0.008 h-1 at 43?C, which was designated to be the critical temperature for this alga strain C1. A suddenly increasing in light intensity from 100 to 500 ?E/m2/s showed an impact to the cell growth, which it had significant affects to photosynthesis (as O2-evolution) as well as its pigment content (chlorophyll and phycocyanin). However, a less effect on the alga growth was obtained when the cells were shifted to grow at higher temperature of 43?C. The alga cells grown at high temperature with high light intensity resulted in decreasing protein content while carbohydrate content increased. The fatty acid profiles of the cells grown at high temperature exhibited a decrease in polyunsaturated fatty acid (C18:3?6,9,12) while C18:2?9,12 increased. In contrast, the alga cells grown in a high light intensity showed an increase in
C18:2?9,12, while C18:1?9 and total fatty acid content (TFA) were decreased. On the other hand, the combined effects of temperature and light intensity (43?C, 500 ?E/m2/s) showed that the period of recovery in photosynthesis depends upon the period of exposure to the stress parameter. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Anti HSV-1 Activity of Spirulina platensis Polysaccharide) ผู้เขียน:Nattayaporn Chirasuwan, Ratana Chaiklahan, Marasri Ruengjitchatchawalya, Boosya Bunnag, นางมรกต ตันติเจริญ สื่อสิ่งพิมพ์:pdf AbstractAqueous extracts of Spirulina platensis were precipitated by cetyltrimethylammonium bromide (CTAB). The hot water extract was found anti Herpes simplex virus type 1 (HSV-1) activity at 50% inhibitory concentration (IC50) values of 21.32 ?g/ml. Partial purification by gel filtration of the crude extract on Sepharose 6B column gave two fractions, SHP-F1 and SHP-F2, which revealed about 4 and 2 times higher activity than that of the crude hot water extract, respectively. The crude hot water extract was a polysaccharide with rhamnose as the main sugar component. Calcium ion and sulfate groups in this polysaccharide had major roles in antiviral activity. However, the crude hot water extract polysaccharide contained approximately 42% carbohydrate and 31% protein. Decreasing the amount of protein by precipitation with trichloroacetic acid (TCA) resulted in higher purity of the crude hot water extract polysaccharide. |