Search Result of "R. Kuhapituktum"

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Agrobacterium Mediated Transformation of Sri Sumrong 60, a Thai Cotton Variety)

ผู้เขียน:ImgH. Chair, ImgR. Kuhapituktum, ImgT. Attathom, ImgC. Pannetier, ImgS. Attathom



Techniques were developed for the induction of embryogenic callus, as well as the transformation and regeneration of Sri Sumrong 60, a Thai cotton variety. Somatic embryogenesis was obtained by culturing hypocotyls explants on MS based medium containing zeatin and NAA and subculturing on an hormone-free medium. This method yielded 23-50% embryogenic callus induction. Transformation of Sri Sumrong 60 with a synthetic CryIA (b) gene was achieved by using Agrobacterium tumefaciens strain, with the addition of 100 mM acetosyringone to the medium during inoculation and co-cultivation steps. PCR amplification was used to verify the presence of cryIA (b) in regenerated plants, thus suggesting the successful transformation of the Thai cotton variety Sri Sumrong 60.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 031, Issue 5, Jan 97 - Dec 97, Page 149 - 156 |  PDF |  Page