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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Detection of Quality Protein Maize (QPM) using Simple Sequence Repeat (SSR) Markers and Analysis of Tryptophan Content in Endosperm) ผู้เขียน: ดร.พีรนุช จอมพุก, รองศาสตราจารย์ , ดร.วาสนา วงษ์ใหญ่, รองศาสตราจารย์ , ดร.ชบา จำปาทอง,  Somsak Apisitvanich สื่อสิ่งพิมพ์:pdf AbstractQuality protein maize (QPM) controlled by opaque-2 (o2) gene was detected using two simple sequence repeat (SSR) markers and further confirmed by tryptophan content in the endosperm. Three populations, i.e., Pop61C1, Pop62C6 and Pop65C6 from the International Maize and Wheat Improvement Center (CIMMYT) were used. S0-plants were selected for the presence of QPM using two markers (phi057 and phi112) as indicated by the amplified products of 140-160 bp. The phi057 marker identified 24 out of 40 Pop61C1 plants (60%), 34 out of 35 Pop62C6 plants (97%) and 24 out of 30 Pop65C6 plants (80%) to be opaque-2 positive while phi112 marker identified 34 (85%), 35 (100%) and 30 (100%) to be opaque-2 plants from the respective populations. Since phi112 was a dominant marker not for detecting the heterozygous genotype, it could only indicate the difference between QPM inbreds and normal maize. The phi057, on the other hand, is a co-dominant marker and could identify the heterozygote of maize plants and therefore, the contamination of non-QPM presented in CIMMYT population. The endosperms of selected S1-seed were further analyzed for the tryptophan and protein content. The three maize populations were found containing the same protein quantity, but different in tryptophan content. The average tryptophan contents in endosperm of QPM and non-QPM as detected by phi057 marker were 0.66% (for QPM), 0.38% (for non-QPM), 0.38% for Suwan 1 (a non-QPM) and 0.80% for the opaque-2 control variety. Moreover, those QPM and non-QPM plants detected by phi112 showed the same result of total protein and tryptophan content in endosperm. To detect heterozygote maize for backcross breeding program, phi057 was considered more feasible than phi112 as a marker assisted selection (MAS) for opaque-2 and to identify QPM line in the short period of time. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Combining Ability of Inbred Lines Derived from Quality Protein Maize Populations) ผู้เขียน:ดร.พีรนุช จอมพุก, รองศาสตราจารย์, ดร.วาสนา วงษ์ใหญ่, รองศาสตราจารย์, Somsak Apisitvanich, ดร.ชบา จำปาทอง สื่อสิ่งพิมพ์:pdf AbstractQuality protein maize (QPM) with improved kernel quality over o2o2 soft genotypes was developed by introducing modifier genes and selecting for a hard, vitreous endosperm in o2o2 germplasm at the International Maize and Wheat Research Center (CIMMYT). Three QPM populations developed by CIMMYT were used in the research. S0-plants of preferred morphological characters were selfpollinated to produce S1 until S3 lines. Ten S3 inbred lines were examined for the opaque-2 gene using the phi057 marker and a diallel cross was made. The experiment was conducted in a 7?7 simple lattice design at the National Corn and Sorghum Research Center, Thailand. The results showed that 10 inbred lines were detected with polymorphism the same as opaque-2 maize (o2o2) but were different from nonopaque- 2 maize. The protein content in endosperm of these inbred lines ranged from 7.76 to 8.61% while those of opaque-2 and non-opaque-2 maize contained about 8.45 and 8.73%, respectively. However, the protein content of inbred lines, the diallel cross and check variety were not significantly different. Means of grain yield (t ha-1) were 4.48 for the diallel cross (F1), 1.97 for inbred lines, 3.30 for opaque- 2 and 6.61 for non-opaque-2 hybrid check. However, grain yield of the best diallel cross was 6.10 t ha-1 which was not significantly different from the non-opaque-2 hybrid. Protein contents were not significant among the diallel cross, opaque-2 and non- opaque-2 hybrids. Tryptophan content in endosperm of the diallel cross was higher than non-opaque-2 hybrids. Inbred P10 had the best combination of GCA effects for grain yield. Moreover, inbred lines, P1, P7, P8 and P9 also gave positive GCA effects. |
ที่มา:Kasetsart Journal (Natural Science)(วารสารวิทยาสารเกษตรศาสตร์ สาขาวิทยาศาสตร์)หัวเรื่อง:Combining ability of inbred lines derived from quality protein maize populations |
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