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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์


ผู้เขียน:ImgPornchai Chuthamas, Imgนางสาวปรานอม พฤฒพงษ์, ผู้ช่วยศาสตราจารย์, Imgนายอิศรา วงศ์ข้าหลวง, รองศาสตราจารย์, Imgสุรียา ตันติวิวัฒน์, ImgManuwadee Ngaosuwan, ImgPiyarat Parinyapong



Tissue culture techniques for propagation and conservating of Wai Takra Thong ( Calamus caesius Blume.) has been carried out at the Royal Chitralada Projects and Department of Horticulture, Kasetsart University. Attempts have been made to induce callus and plant regeneration from immature embryo of Wai Takra Thong. The immature embryos were cultured on the MS (Marashige and Skoog, 1962) medium to obtain clean culture of explants. Cut the explants into thin pieces and culture on MS, Y3 (Eeuwens, 1976) and WPM (Lloyd McCown, 1981) media additional with various concentrations of 2,4-D and BAP in plastic well plate. The culture on both MS media with 0.5 mg/l 2,4-D + 2 mg/l BAP and 0.1 mg/l 2,4-D + 4 mg/l BAP produced callus and multiplication. Shoot and root formation appeared on the same media.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 025, Issue 3, Jul 91 - Sep 91, Page 299 - 304 |  PDF |  Page