Search Result of "Phimchanok Nakkharat"

About 1 results

ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Characterization of a Crude Thermostable ?-galactosidase by the Bacterium PD1 Isolated from the Pong Dueat Hot Spring)

ผู้เขียน:ImgPhimchanok Nakkharat, ImgArkom Tesnum, ImgArkira Maethawarakorn, ImgDietmar Haltrich, Imgนางจิรกานต์ เมืองนาโพธิ์



?-Galactosidase is an enzyme that hydrolyses lactose to glucose and galactose. The present study focused on the characterization of ?-galactosidase from a thermophilic microoganism isolated from the Pong Dueat hot spring, Chaingmai Province, Thailand. o-Nitrophenyl-L-D-galactopyranoside (oNPG) was used as a substrate to determine the ?-galactosidase activity from a strain PD1, which was found to be the active strain producing the highest ?-galactosidase activity. The optimum pH and temperature for enzyme activity were 6.5 and 55?C, respectively. The enzyme was stable at pH range of 6 to 10. The half-life of enzyme at 80, 70, 60, 55, 50 and 45?C were 0.5, 2, 31, 62, 87 and 435 h, respectively. The Km and Vmax values for the synthetic substrate oNPG were 11 mM and 1.1?10-2 mmolL-1min-1, respectively.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 042, Issue 5, Dec 99 - Dec 99, Page 264 - 268 |  PDF |  Page