 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : RAPD Analysis for Varietal Identification in Brassica) ผู้เขียน: Panie Temiesakl, Yauwanit Ponpimt, Takeo Harada สื่อสิ่งพิมพ์:pdf Abstract The concentration of DNA extracted from leaves of Brassica seedlings was amplified by RAPD (Random Amplified Polymorphic DNA) using 35 single 10-base oligonucleotide primers to analyzed genetic relationships among Brassica species. Amplified products were then analyzed by polyacrylamide gel electrophoresis. Each species expressed specific banding pattern. RAPD products were then used to (a) prove genetic basis of an interspeeific hybrid betweenBrassica campestris andB. juncia, and (b) examine differences both within and among parents and hybrids in B. campestris var. pekinensis. |