 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Chemical, Biochemical and Physical Treatments on the Kinetics and on the Role of Some Endogenous Enzymes Action of Baker’s Yeast Lysis for Food-Grade Yeast Extrac ผู้เขียน: S. Boonraeng, P. Foo-trakul, W. Kanlayakrit, C. Chetanachitra สื่อสิ่งพิมพ์:pdf AbstractThe effects of chemical, biochemical and physical treatments on lysis of baker’s yeast cells were studied at pH 5.2 and temperature 54 ?C. An unstructured kinetic model of first order was developed to understand the mechanism of yeast lysis. The autolysis rate constant (k), yield factor for protein (?P), total carbohydrate (?C) and autolysate (?A) were sensitive to disruption methods. Since there were endogenous enzymes in the yeast cells such as proteinases, glucanases and carboxypeptidases, these enzymes were responsible for synergistic of cell disruption and their activity profiles were affected by different treating methods. From the determined results of amino acids composition in yeast autolysate, there were differences in amino acid content. The biochemical treated sample using 0.1% papain gave the highest total amino acid content among all analysed samples. |