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ที่มา:การประชุมวิชาการพืชสวนแห่งชาติ ครั้งที่ 10หัวเรื่อง:ผลของสารโคลชิซินและ oryzalin ต่อการชักนำต้นดับเบิลแฮพลอยด์ในการเพาะเลี้ยงอับละอองเกสรพริก |
ที่มา:การประชุมวิชาการพฤกษศาสตร์แห่งประเทศไทย ครั้งที่ 5หัวเรื่อง:การเพิ่มจำนวนชุดโครโมโซมต้นแตงกวาแฮพลอยด์โดยใช้สาร oryzalin |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : In Vitro Chromosome Doubling in Korarima [Aframomum corrorima (Braun) P.C.M. Jansen] Using Colchicine and Oryzalin) ผู้เขียน: ดร.สุรวิช วรรณไกรโรจน์, รองศาสตราจารย์ ,  Wondyifraw Tefera สื่อสิ่งพิมพ์:pdf AbstractPloidy manipulation holds high promise for the improvement of korarima (Aframomum corrorima) plants with altered morphology, as well as of potentially altered essential oil yields and oil composition. The present work aimed to establish a protocol for in vitro polyploid induction of korarima. Nodal explants 2 mm long were cultured in liquid media with 0, 125, 250, 375 or 500 ?M colchicine or 10, 20, 40, 60 or 80 ?M oryzalin for 7 d. The lowest concentration was the most effective for polyploid induction with both colchicine and oryzalin. Teteraploid plants were obtained only from shoots regenerated from explants treated with 125 ?M colchicine and 10 ?M oryzalin. Oryzalin was thus confirmed to be effective at a lower concentration than colchicine. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Some Chemical Treatments on Kluai Khai Through Tissue Culture for Mutation Breeding) ผู้เขียน:ดร.ภาสันต์ ศารทูลทัต, ผู้ช่วยศาสตราจารย์ , นางเบญจมาศ ศิลาย้อย, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractPolyploidy of in vitro Kluai Khai plantlets were induced by different concentrations of the two mutagens : 0, 0.5, 0.75 and 1% colchicine and 0,15, 30, 45mM oryzalin containing 2% DMSO for 2.5, 5.0, 7.5 hours. It was found that the higher the mutagen doses and the longer the treatment duration, the less the survival rates. After treating plantlets with colchicine and oryzalin at 0.4, 0.5, 0.75% and 13, 15, 22mM, respectively the survival rate of each one was found to be 50%. In MV1, adventitious bud initiation of mutagenic treatment yielded 0.2-1.2 shoots which was lower than those in the control while those of MV2 and MV3 were not significantly different from each other. Treated plantlets were revealed to be abnormal in chimera and stomata sizes were larger than those of the controls. The plantlets with stomata over 28mm were selected for chomosome count. The controls were diploid (2n = 22) whereas three selected clones were tetraploid (2n = 44) derived from 1% colchicine at 7.5 hours and 45mM oryzalin at 2.5 hours. The mutants were then subcultured. The number of suckers of mutants was less than those of the control. MS medium without plant growth regulator was observed to give the best rooting of all treatments. After transfered to the greenhouse, the height of the controls and the colchicine treated plants were revealed to be significantly different from the oryzalin treated ones which were shorter with low survival rate. The results obtained in the field were similar to those in the greenhouse. The foliage of all mutants appeared more drooping with larger stomata cell, lower in stomata number per area and thicker than those of the controls. The numbers of leaf and sucker of each mutant were not significantly different from the controls. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Petal Color and Petal Form Mutations Observed in Torenia hybrida Following Gamma Irradiation in vitro) ผู้เขียน:Valerie W. Suwanseree, Tharathorn Teerakathiti, ดร.เฌอมาลย์ วงศ์ชาวจันท์, ผู้ช่วยศาสตราจารย์ , นายธัญญะ เตชะศีลพิทักษ์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe aim of this study was to develop new cultivars of Torenia hybrida for the ornamental market. Excised nodes of T. hybrida plantlets grown in vitro were exposed to 0, 0.0025, 0.0075 or 0.0125 mM colchicine or 0, 0.0028 mM, 0.0086 mM or 0.144 mM oryzalin for 48 or 72 h before subsequent in vitro multiplication. Four-week-old in vitro plantlets from the colchicine treatments were irradiated with 0, 30, 40, or 50 Gy of gamma radiation and those from the oryzalin treatments were irradiated with 0 or 60 Gy of gamma radiation. After subculturing, the plants were transferred to the field and changes in phenotype were noted. Colchicine and oryzalin treatment did not result in any polyploid plants. No variations were observed in leaf shape, color or size but variations were observed in growth habit (compact and creeping), flower color (pink and pale blue, as well as mottled or streaked purple petals), and flower form (erose petal margins). The plants with erose petal margins were selected for possible development of a new cultivar. |
ที่มา:การประชุมทางวิชาการของมหาวิทยาลัยเกษตรศาสตร์ ครั้งที่ 47หัวเรื่อง:ผลของออริซาลินที่มีต่อลักษณะทางสัณฐานวิทยาของกล้วยหิน |
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