Search Result of "Nongluck Ruangwises"

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ที่มา:วารสาร คณะสัตวแพทยศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Antibacterial Activity of Crude Extracts of Centella asiatica against Staphylococcus aureus in Bovine Mastitis)

ผู้เขียน:Imgดวงกมล แต้มช่วย, Imgดร.ธีระ รักความสุข, รองศาสตราจารย์, Imgดร.ธวัชชัย ศักดิ์ภู่อร่าม, ผู้ช่วยศาสตราจารย์, ImgNongluck Ruangwises



Crude extracts of Asiatic Pennywort (Centella asiatica (Linn.) Urban) extracted with either ethannol or water were tested for antibacterial activity against 30 isolates of S. aureus from milk samples of dairy cows. The antibacterial sensityvity of crude extracts were tested by the disc diffusion test, and results showed that ethanol extracts and water extracts had average inhibition zones ranged from 6.44-6.49 and 6.54-17.72 mm in diameter, respectively .The modified resazurin microtiter-plate was used to determine the minimum inhibitory concentration (MIC) , and the minimum bactericidal concentration (MBC) was determined by touching the loop from each well of MIC plate and streaking it on a mannitol salt agar. Results showed that the ethanol extracts had an MIC 50 value of 8 mg/mL, the water extracts of leaf powder had an MIC50 value of 32 mg/mL, and the water extracts of fresh leaves had an MIC value of 32-256 mg/mL. The ethanol extracts had an MBC value of 16 mg/mL. The water extracts could not kill S. aureus. In conclusion, the ethanol extracts had more potential antibacterial activity than the water extracts.

Article Info
วารสาร คณะสัตวแพทยศาสตร์(Journal of Kasetsart Veterinarians), Volume 019, Issue 3, Sep 09 - Dec 09, Page 119 - 128 |  PDF |  Page