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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์


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Sugarcane shoot tips were cultured in liquid media continuously stirred in circular manner by a gyratory shaker rotated at 2 cycles/min under continuous illumination of 2000-4000 lux. It was found that the MS me?dium supplemented with 0.2 mg/1 BA and 0.02 mg/1 NAA gave the best proliferation rate, 4-5 shootlets could be obtained within 20 days from development of axillary buds. After 50 days the shootlets were multiplied at the rate of 4 folds in every 2 weeks by subculturing into the same medium The resulting shootlets could be progres?sively increased at the rate of 6 folds in every 4 weeks when transferred on to an MS solid medium, supple?mented with 0.2 mg/l BA and 0.02 mg/1 NAA before transferred on to MS medium containing 15oh (v/v) coco?nut water and 1 mg/ l 2,4-D to enhance the shoots. Rooting of the shoots were successfully done on a MS me?dium with 1 mg/I IBA.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 027, Issue 3, Jul 93 - Sep 93, Page 286 - 291 |  PDF |  Page