 หัวเรื่อง:การเพาะเลี้ยงเนื้อเยื่อปลายยอดอ้อยให้พัฒนาเป็นต้นพืชที่สมบูรณ์ ผู้เขียน: Kriuk Naritoom,  นายเกษม สุขสถาน, ผู้ช่วยศาสตราจารย์ , Vichai Korpradittskul, Isara Suksathan, Rongrong Visessuwan, ดร.นงณพชร คุณากร , นางสุภาพร กลิ่นคง, รองศาสตราจารย์ , Monthar Suriyachaiyakorn สื่อสิ่งพิมพ์:pdf AbstractSugarcane shoot tips were cultured in liquid media continuously stirred in circular manner by a gyratory shaker rotated at 2 cycles/min under continuous illumination of 2000-4000 lux. It was found that the MS me?dium supplemented with 0.2 mg/1 BA and 0.02 mg/1 NAA gave the best proliferation rate, 4-5 shootlets could be obtained within 20 days from development of axillary buds. After 50 days the shootlets were multiplied at the rate of 4 folds in every 2 weeks by subculturing into the same medium The resulting shootlets could be progres?sively increased at the rate of 6 folds in every 4 weeks when transferred on to an MS solid medium, supple?mented with 0.2 mg/l BA and 0.02 mg/1 NAA before transferred on to MS medium containing 15oh (v/v) coco?nut water and 1 mg/ l 2,4-D to enhance the shoots. Rooting of the shoots were successfully done on a MS me?dium with 1 mg/I IBA. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : In vitro Preservation of Sugarcane Callus under Low Temperature) ผู้เขียน:ดร.เรวัต เลิศฤทัยโยธิน, รองศาสตราจารย์ , Rongrong Visessuwan, Kriuk Naritoom สื่อสิ่งพิมพ์:pdf AbstractCallus was induced from commercial cultivar of sugarcane by culture shoot tip on Murashige and Skoog medium supplemented with 3 mg/1/2, 4-dichlorophenoxyacetic acid and 15% by volume of coconut water. This callus was preserved under low temperature at 1 and 5 degree C with different periods of preservation, and then was cultured at 27 degree C for 6 months. After preservation under low temperature, plantlets could be induced from callus preserved at 5 degree C but could not be induced from callus preserved at 0 degree C. In addition, at 5 degree C callus could develop to be plantlets only when preserved not longer than 30 days. Callus preserved at low temperature grew slower than callus not preserved at low temperature. |
หัวเรื่อง:การคัดเลือกพันธุ์กลายต้นเตี้ย อายุสั้น และผลผลิตสูงในถั่วเหลืองที่ผ่านการฉายรังสีแกมมา ผู้เขียน:นางอรุณี วงศ์ปิยะสถิตย์, ศาสตราจารย์, นายสุมินทร์ สมุทคุปต์, ศาสตราจารย์, นางสิรนุช ลามศรีจันทร์, ศาสตราจารย์, Kriuk Naritoom, Ekapol Junkhunthod สื่อสิ่งพิมพ์:pdf AbstractPreliminary yield trail of 7 selected soybean lines (line numbers 2035, 2037, 2125, 2156, 2160, 2180 and 2192) obtained from crosses between mutants and Doi Kham variety and one line (line number 58608) obtained from gamma irradiated Doi Kham along with four check varieties (Doi Kham, S.J.4, S.J. 5 and CM.60) was carried out in order to select for short stature, early maturity and high yielding mutant. Line numbers 2156, 58608, 2160 and 2180 expressed short stature and early maturity. Their heights at maturity time were shorter that the original Doi Kham for 13, 15, 17 and 22 cm respectively, and their yields were 13, 11, 11 and 13 days earlier than Doi Kham, respectively. Their yields were similar to Doi Kham. These four lines will be further tested at different locations in the dry and rainy seasons. The best line will be selected and used as a variety. |