Search Result of "Kasemsak Palakorn"

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Isozymes Electrophoresis for Varietal Verification in Some Tropical Vegetables )

ผู้เขียน:ImgPanic Temiesak, ImgKasemsak Palakorn, ImgKarnda Tantiyavarong, Imgนายพรพันธ์ ภู่พร้อมพันธุ์

สื่อสิ่งพิมพ์:pdf

Abstract

Techniques for varietal identification of vegetable crops using seed protein and isozymes electrophoresis were investigated. Several isozymes namely, alcohol dehydrogenase (ADH), peroxidase, esterase, glutamic oxaloacetic transaminase (GOT), phosphoglucomutase (PGM) and malate dehydrogenase (MDH) were analysed to standardize in proper condition of plant ages, plant parts, sample extraction solution, gel concentration and staining solution. It had been found that 12 % gel was suitable to analyse isozymes and 13.5 % for protein. Isozymes extracted with phosphate buffer, pH 7.5-8.5 from leaves of 7 day old seedling showed the best expression of banding pattern and distinguish the difference among breeding lines, namely peroxidase in sweet corn, ADH, PGM and esterase in Brassica; esterase in tomato and okra

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 027, Issue 5, Jan 93 - Dec 93, Page 43 - 51 |  PDF |  Page