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ผลงานตีพิมพ์ในวารสารวิชาการImproving the nutritive value of mulberry leaves, Morus spp. (Rosales: Moraceae) for silkworm larvae, Bombyx mori (Lepidoptera: Bombycidae) using gamma irradiationผู้แต่ง: Pipatpong Chundang, Karun Thongprajukaew,  Dr.Uthaiwan Kovitvadhi, Associate Professor , Banthari Chotimanothum, Dr.ATTAWIT KOVITVADHI, Assistant Professor , Mrs.Pannee Pakkong, Associate Professor , วารสาร: |
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 การประชุมวิชาการแมลงชนิดใดดีที่สุดสำหรับเป็นอาหารเป็ดเนื้อจากผลการย่อยได้ในหลอดทดลอง (2019)ผู้แต่ง:Suwanan Kerttewa, Paksupa Pleehajinda, Nuttika Deethong, Pipatpong Chundang, Karun Thongprajukaew, Chanin Tirawattanawanich, Sunyanee Srikachar, Banthari Chotimanothum, Dr.ATTAWIT KOVITVADHI, Assistant Professor, การประชุมวิชาการ: |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Characterization and Expression Levels of Protease Enzymes at Different Developmental Stages of Siamese Fighting Fish (Betta splendens Regan, 1910)) ผู้เขียน:Karun Thongprajukaew, ดร.อุทัยวรรณ โกวิทวที, รองศาสตราจารย์, ดร.อรุณี อิงคากุล, ผู้ช่วยศาสตราจารย์ , Krisna Rungruangsak-Torrissen สื่อสิ่งพิมพ์:pdf AbstractCharacterization of total proteases, trypsin and chymotrypsin were performed at pH 2 12 and temperatures 20-80?C in three life stages of Siamese fighting fish (Betta splendens Regan, 1910). At least fourteen protease activities were detected, including seven acidic (pH levels 2, 4 and 5-6), one neutral (pH 7), and six alkaline (pH levels 8-12) activities in the optimal temperature range of 30-60?C. The neutral and alkaline pH profiles were different between sexes during the on-growing stage (1.5 month-old). The acidic proteases played a major role in digestion in the early life stage (10-day-old), while the alkaline proteases became more important toward older stages. Trypsin and chymotrypsin showed similar characteristics with at least five activities observed. For trypsin, activity was detected with pH 7 and 8 at 50?C, and pH 10 at 30, 50 and 60?C. For chymotrypsin, activity was found at pH levels 7 and 8 at 50?C, and pH 10 at 30, 40 and 50?C. Regardless of sex and age, the most suitable conditions for studying digestive enzyme activities in general were at pH 8 and 50?C for total proteases, pH 7-8 and 50?C or pH 10 and 30-35?C for trypsin and pH 7-8 and 50?C or pH 10 and 40?C for chymotrypsin. Trypsin and chymotrypsin are important proteases showing similar general optimal conditions to that of total proteases. This information provides elementary knowledge for studying protein digestibility by in vitro techniques and for determining growth performance quality using digestive enzyme markers in Siamese fighting fish. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Temperature and pH Characteristics of Amylase and Lipase at Different Developmental Stages of Siamese Fighting Fish (Betta splendens Regan, 1910)) ผู้เขียน:Karun Thongprajukaew, ดร.อุทัยวรรณ โกวิทวที, รองศาสตราจารย์, ดร.อรุณี อิงคากุล, ผู้ช่วยศาสตราจารย์, Krisna Rungruangsak-Torrissen สื่อสิ่งพิมพ์:pdf AbstractTemperature (20-80?C) and pH (2-12) profiles of amylase and lipase at three stages (10-dayold, 1.5-month-old and 3-month-old) in Siamese fighting fish (Betta splendens Regan, 1910) were studied. At least nine amylase activities were observed during development: pH 7 at 40?C, pH 9 at 30?C and 50? C, pH 8 and pH 11 at 50?C in females and pH 8 and pH 11 at 40?C and 60?C in males. At the younger stage (? 1.5 month old), activity at pH 7 and 40?C, and at pH 9 and 30?C and 50?C was observed. At the older stage (? 1.5 month old), amylase activity at pH 8 and pH 11 in the temperature range of 40-60?C was found. Lipase had at least five levels of activity: pH 7 at 20?C and 40?C, pH 8 at 20-40?C and 60?C, and at pH 11, where the activity peak disappeared in the maturing stage (3 month old) in both sexes. Amylase had very low specific activity at the 10-day-old stage, while lipase had a high specific activity, similar to older stages. The most suitable pH to determine amylase and lipase activity in Siamese fighting fish was pH 8, whereas a temperature of 50?C was appropriate for amylase and 40?C was suitable for lipase, regardless of sex and age. This information is a prerequisite for future studies of the in vitro digestibility evaluation of nutrient utilization in Siamese fighting fish. |
Researcherดร. อุทัยวรรณ โกวิทวที, รองศาสตราจารย์ที่ทำงาน:ภาควิชาสัตววิทยา คณะวิทยาศาสตร์ บางเขน สาขาที่สนใจ:Ecology, Taxonomy, Culture Gloehidia in Modified Media nad Rearing Adult of Freshwater Mussel Freshwater Ecology, Aquaculture Resume |
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