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 ผลงานตีพิมพ์ในวารสารวิชาการUltrastructural alterations of frozen-thawed Asian elephant (Elephas maximus) spermatozoaผู้แต่ง: Sa-Ardrit M., Saikhun J.,  Dr.Nikorn Thongthip, Associate Professor , Mr.Kornchai (Mangkorn) Kornkaewrat (Damyang), Mahasawangkul S., Angkawanish T., Jansittiwate S., Faisaikarm T., Kitiyanant Y., Pavasuthipaisit K., Dr.Anuchai Pinyopummin, Associate Professor, วารสาร:  
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ผลงานตีพิมพ์ในวารสารวิชาการPotential factors affecting semen quality in the Asian elephant (Elephas maximus)ผู้แต่ง:Dr.Nikorn Thongthip, Associate Professor, Saikhun, J, Mahasawangkul, S, Mr.Kornchai (Mangkorn) Kornkaewrat (Damyang), Pongsopavijitr, P, Songsasen, N, Dr.Anuchai Pinyopummin, Associate Professor, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการSemen characteristics and sperm morphology of serow (Capricornis sumatraensis)ผู้แต่ง:Suwanpugdee, A., Kornkeawrat, K., Saikhun, K., Siriaroonrat, B., Tipkantha, W., Doungsa-ard, K., Sa-ardrit, M., Suthunmapinatha, P., Dr.Anuchai Pinyopummin, Associate Professor, วารสาร:  |
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ผลงานตีพิมพ์ในวารสารวิชาการSeminal Plasma MDA Concentrations Correlating Negatively with Semen Quality in Asian elephantsผู้แต่ง:Dr.Nikorn Thongthip, Associate Professor, Imrat, P, Srihawong, T, Mahasawangkul, S, Dr.Chanin Tirawattanawanich, Assistant Professor , Saikhun, K, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการAssessment of viability and acrosomal status of Asian elephant (Elephas maximus) sperm after treatment with calcium ionophore and heparinผู้แต่ง:Sa-ardrit, M, Dr.Nikorn Thongthip, Associate Professor, Mr.Kornchai (Mangkorn) Kornkaewrat (Damyang), Faisaikarm, T, Kitiyanant, Y, Mahasawangkul, S, Dr.Anuchai Pinyopummin, Associate Professor, Saikhun, K, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการNuclear maturation and development of IVM/IVF canine embryos in synthetic oviductal fluid or in co-culture with buffalo rat liver cellsผู้แต่ง:Saikhun, J, Sriussadaporn, S, Dr.Nikorn Thongthip, Associate Professor, Dr.Anuchai Pinyopummin, Associate Professor, Kitiyanant, Y, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการRelationship between seminal and serum calcium concentration with semen quality in the Asian elephant (Elephas maximus)ผู้แต่ง:Sivilaikul, S., Jitprom, A., Kularb, A., Kornkaewrut, K., Suthanmaphinuth, P., Mahasawangkul, S., Saikhun, K., Wajjwalku, W., Dr.Nikorn Thongthip, Associate Professor, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการSuccessful artificial insemination in the Asian elephant (Elephas maximus) using chilled and frozen-thawed semenผู้แต่ง:Dr.Nikorn Thongthip, Associate Professor, Mahasawangkul, S, Thitaram, C, Pongsopavijitr, P, Mr.Kornchai (Mangkorn) Kornkaewrat (Damyang), Dr.Anuchai Pinyopummin, Associate Professor, Angkawanish, T, Jansittiwate, S, Rungsri, R, Boonprasert, K, Wongkalasin, W, Homkong, P, Dejchaisri, S, Dr.Worawidh Wajjwalku, Associate Professor, Saikhun, K, วารสาร: |
ผลงานตีพิมพ์ในวารสารวิชาการEffect of pre-freeze semen quality, extender and cryoprotectant on the post-thaw quality of Asian elephant (Elephas maximus indicus) semenผู้แต่ง:P. Imrat, Ms.Piyawan Suthunmapinanta , K. Saikhun, S. Mahasawangkul, E. Sostaric, P. Sombutputorn, S. Jansittiwate, Dr.Nikorn Thongthip, Associate Professor, Dr.Anuchai Pinyopummin, Associate Professor, B. Colenbrander, W.V. Holt, T.A.E. Stout, วารสาร: |
 ที่มา:วารสาร คณะสัตวแพทยศาสตร์หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Quality of Bull Deep Frozen Semen Extended with Different Glycerol Concentrations: Evaluated by Computer Assisted Semen Analysis) ผู้เขียน:Rapiphan Uavechanichkul, Jatuporn Pongpeng, Sinchai Wirojwutthikul, Lertchai Jintapitaksakul, Kunnasant Saikhun, Mukda Rattanapaskorn สื่อสิ่งพิมพ์:pdf AbstractQuality of bull deep frozen semen extended with 87% and 99% glycerol in egg yolk tris extender with one-step and two-step dilution methods assessed by CASA (Computer Assister Semen Analysis) were compared. Semen of 20 Holstein-Fresian bulls aged 3-5 years were collected and frozen once a week for 3 weeks. The result showed the post-thaw sperm motility and progressive motility at 0 h from the 87% and 99% glycerol were not significantly different (P>0.05). The ALH (amplitude of lateral head displacement) and BCF (beat cross frequency) of the sperm from the extender using the 99% glycerol was significantly higher (P<0.05). However, after 5 h of incubation at 37?C the 87% glycerol gave significantly lower motility and progressive motiloty with higher VAP (average path velocity), VCL (curvilinear velocity) and ALH (P<0.05). The results of dilution methods did not differ significantly at 0 h. except at 5 h. the one-step method revealed significant higher motility (P<0.05. There were no effects of glycerol cencentration and dilution methods on post-thaw sperm head elongation, area, plasma membrane and acrosomal integrity (P>0.05). In conclusion, using 99% glycerol and one-step dilution methods gave a better semen quality. |
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Semen Quality and Chromatin Condensation in Domestic Cat Sperm During Passage Through the Epididymis) ผู้เขียน:Sarawut Sringam, Yindee Kitiyanant, Lawrence M. Lewin, Kulnasan Saikhun สื่อสิ่งพิมพ์:pdf AbstractThe objectives were to identify and quantify changes occurring in cat sperm as they pass through the epididymis, vas deferens and ejaculate. Cat epididymides obtained by orchidectomy were divided into six regions (1 to 6) and sperm cells were released by mincing each epididymal region. The semen quality was assessed by light microscopy and the sperm chromatin condensation was measured by flow cytometry. The sperm motility was minimal in region 1 and increased significantly (p < 0.05) when the sperm passed from this region, with maximum motility reached in region 6, the vas deferens and ejaculate. Progressive motility and the percentage of sperm with normal morphology increased during epididymal transit. The most prevalent defects in abnormal spermatozoa were found in the tail. The mean degree of maturation (? standard error of the mean) of sperm chromatin condensation was 72.9 ? 9.4% in region 1 and was significantly (p < 0.05) increased in region 3 (94.4 ? 2.6%). Chromatin condensation or decondensation processes were studied further in cat epididymides (four portions: initial segment, caput, corpus and cauda) by incubating sperm with alkaline phosphatase (AP) or dithiothreitol (DTT), respectively, followed by staining with propidium iodide and analysis by flow cytometry. After AP treatment, the fluorescence intensity approximated that found in the initial segment. The DTT treatment significantly (p < 0.05) increased the fluorescence of the chromatin condensation in all parts, except the initial segment. Cat sperm chromatin was shown to undergo condensation during passage through the epididymis and the condensation process was reversed by reducing disulfide to sulfhydryl bonds or promoted by dephosphorylation. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Extender and Storage Time on Motility and Ultrastructure of Cooled-Preserved Boar Spermatozoa) ผู้เขียน:Duangjai Boonkusol, Kulnasan Saikhun, Poonsuk Ratanaphumma สื่อสิ่งพิมพ์:pdf AbstractArtificial insemination (AI) using fresh diluted semen is used worldwide in the porcine industry. Viability of spermatozoa in diluted semen depends on several factors, such as the interaction with the type of extender and storage duration. The aim of this study was to evaluate the effects of extenders and storage time on the motility and ultrastructure of cooled-preserved boar spermatozoa. Semen samples were collected, diluted in BTS, Merck III or Androhep and stored at 15?C for 0, 1, 3, 5 and 7 d. The samples were warmed (37?C) and spermatozoa were evaluated for motility using light microscopy and ultrastructure using scanning (SEM) and transmission (TEM) electron microscopy. The results showed that motility did not differ significantly (P>0.05) among extenders at days 0 to 3, while Androhep showed a significantly (P<0.05) higher percentage of motility than BTS and Merck III from days 5 to 7. At day 7 of semen storage, plasma and acrosomal membrane damage were observed, as revealed by SEM and TEM. These findings suggest that the type of semen extender and cooled-preserved time affected motility and caused structural damage to boar spermatozoa, especially in the plasma and acrosomal membrane. |
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