 หัวเรื่อง:การเพาะเลี้ยงเนื้อเยื่อปลายยอดอ้อยให้พัฒนาเป็นต้นพืชที่สมบูรณ์ ผู้เขียน: Kriuk Naritoom,  นายเกษม สุขสถาน, ผู้ช่วยศาสตราจารย์ , Vichai Korpradittskul, Isara Suksathan, Rongrong Visessuwan, ดร.นงณพชร คุณากร , นางสุภาพร กลิ่นคง, รองศาสตราจารย์ , Monthar Suriyachaiyakorn สื่อสิ่งพิมพ์:pdf AbstractSugarcane shoot tips were cultured in liquid media continuously stirred in circular manner by a gyratory shaker rotated at 2 cycles/min under continuous illumination of 2000-4000 lux. It was found that the MS me?dium supplemented with 0.2 mg/1 BA and 0.02 mg/1 NAA gave the best proliferation rate, 4-5 shootlets could be obtained within 20 days from development of axillary buds. After 50 days the shootlets were multiplied at the rate of 4 folds in every 2 weeks by subculturing into the same medium The resulting shootlets could be progres?sively increased at the rate of 6 folds in every 4 weeks when transferred on to an MS solid medium, supple?mented with 0.2 mg/l BA and 0.02 mg/1 NAA before transferred on to MS medium containing 15oh (v/v) coco?nut water and 1 mg/ l 2,4-D to enhance the shoots. Rooting of the shoots were successfully done on a MS me?dium with 1 mg/I IBA. |