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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Toxicity and Genotoxicity of Pendimethalin in Maize and Onion) ผู้เขียน: Nuttapol Promkaew, Puangpaka Soontornchainaksaeng,  ดร.สรรเสริญ จำปาทอง , Piangchan Rojanavipart สื่อสิ่งพิมพ์:pdf AbstractHerbicides are applied widely to maize crops. Frequently, these chemicals cause genetic change. Thus, this study was carried out with the aim to modify the Allium test using maize and onion to evaluate the toxicity and genotoxicity of pendimethalin. The effective concentration (EC), mitotic index (MI) and chromosomal aberrations found in treated root meristems of onion (Allium cepa) and three cultivars of Zea mays (HSSS, Insee2 and Suwan4452) were used as the endpoints of toxic and genotoxic evaluation. The results revealed that pendimethalin affected the toxicity and genotoxicity on three cultivars of maize and on onion. The percentages of recommended dose (7.5 ml/L) of pendimethalin at EC30, EC50, and EC70 were 0.05, 0.08 and 0.15% for HSSS, 0.02, 0.04 and 0.08% for Insee2, 0.02, 0.05 and 0.09% for Suwan4452, and 0.07, 0.16 and 0.29% for onion, respectively. Pendimethalin caused a significant increase in both the mitotic index and the frequency of chromosomal aberrations for all plants in the study. The types of chromosomal aberrations detected in root cells were fragments, bridges, laggards, multipolar and micronuclei. Moreover, abnormal metaphase (C-mitosis) and polyploidy, as well as the accumulation of metaphase cells, were also found in treated root cells. The results of this study will provide additional data on the toxicity and genotoxicity of the herbicide and also assist in environmental pollution monitoring. |
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 ที่มา:การประชุมวิชาการและเสนอผลงานวิจัยพืชเขตร้อนและกึ่งร้อน ครั้งที่ 5หัวเรื่อง:การตรวจสอบความเป็นพิษต่อยีนและฤทธิ์ยับยั้งการกลายพันธุ์ของสารสกัดจากลองกองโดยใช้แบคทีเรีย Salmonella |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Risk Assessment of Triclosan Using Animal Cell Lines) ผู้เขียน:Kalyanee Jirasripongpun, Thanate Wongarethornkul, Sunisa Mulliganavin สื่อสิ่งพิมพ์:pdf AbstractTriclosan is an antimicrobial ingredient used in many health-care products and household items including plastics, textiles and food packaging materials. However, the safety of triclosan has been questioned recently. In this study, cytotoxicity of triclosan to BHK-21, Vero and KB cells were performed by incubating these cells in culture medium containing various concentrations of triclosan for 3 days. Vero and KB cells were more sensitive and suitable as models to evaluate triclosan toxicity. The 50% inhibition concentration (IC50) of triclosan to Vero and KB were at 0.036 and 0.034 mM, respectively, while that of BHK-21 was at higher concentration (0.26 mM). Analyzing for triclosan content in popular use of personal health-care products revealed the maximum triclosan content at 0.27 % (w/w) or 0.023 mM in liquid facial soap. Genotoxicity analysis based on comet assay demonstrated the DNA breakage by triclosan. Its genotoxic potency was significantly increased as the triclosan concentration and the exposure time increased. Exclusion of cell death or cytotoxicity, the genotoxic effect of triclosan in this study could be observed noticeably at the IC20-30 of triclosan after 5 days treatment. Therefore, animal cell lines could be used for primary evaluation on the risk assessment of triclosan. |