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 ผลงานตีพิมพ์ในวารสารวิชาการHistology and Histochemical Distribution of Goblet Cells in the Descending Colonic Epithelium of the Swamp Buffalo (Bubalus bubalis)ผู้แต่ง: Ms.Pakawadee Pongket , Mrs.Maleewan Liumsiricharoen, Associate Professor,  Supalak Romratanapan, Mr.Somchai Pongjunyakul, Associate Professor, Dr.Urai Pongchairerk, Assistant Professor, Dr.Apinun Suprasert, Professor , วารสาร: 
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ผลงานตีพิมพ์ในวารสารวิชาการHistology and Glycoconjugates Histochemistry in the Small and Large Intestinal Epithelium of the Malayan Pangolin, Manis javanicaผู้แต่ง:Dr.Apinun Suprasert, Professor, Mrs.Maleewan Liumsiricharoen, Associate Professor, Ms.Pakawadee Pongket, Dr.Teerasak Prapong, Assistant Professor , Mrs.Apantree Chantong, Mrs.Nathanan Prompa, วารสาร: |
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การประชุมวิชาการจุลกายวิภาคศาสตร์และเนื้อเยื่อเคมีของ goblet cell ในเยื่อบุผิวลำไส้ส่วน descending colon ของกระบือปลัก (Bubalus bubalis) (2009)ผู้แต่ง:Ms.Pakawadee Pongket, Mrs.Maleewan Liumsiricharoen, Associate Professor, Mrs.Suppaluk Romratanapun, Associate Professor , Mr.Somchai Pongjunyakul, Associate Professor, Dr.Urai Pongchairerk, Assistant Professor, Dr.Apinun Suprasert, Professor, การประชุมวิชาการ: |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Characterization of Glycoconjugates Present in Cloacal Epithelium of the Chicken) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, นายบุญธรรม จงเจริญ, รองศาสตราจารย์, Suppaluk Romrattanapun สื่อสิ่งพิมพ์:pdf AbstractThe distribution of glycoconjugates in the chicken cloaca was studied by means of light microscopic histochemical methods. The staining procedures employed were horseradish peroxidase conjugated lectin, Alcian blue (AB) pH 1.0, AB pH 2.5, periodic acid –Schiff (PAS), AB pH 2.5-PAS in combination with enzyme neuraminidase digestion. The lectin used in the present study were peanut agglutinin (PNA), wheat germ agglutinin (WGA), Dolichos biflorus agglutinin (DBA), and Lotus tetragonolobus agglutinin (LTA) The mucosa of both coprodeum and urodeum is covered by simple columnar epithelium in which 2 type of cells are encountered-columnar and goblet cells. In contrast, one layer of only columna cells is found in the mucosa of proctodeum. Tubular glands of proctodeum are commonly provided at submucosal layer. All the goblet cells in coprodeum and urodeum and tubular glands of proctodeum were coloured blue and purple with AB pH 1.0 and AB pH 2.5-PAS, respectively. They were furthermore stained moderately with WGA. Neuraminidase digestion changed the blue coloration with AB pH 2.5 to pale blue. Removal of sialic acid with neuraminidase imparted weak to strong affinity of PNA. Histophysiological significances of glycoconjugates involved in the chicken cloaca were discussed with special reference to the functional activities of the carbohydrates. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Lectin Histochemical Characterization of Glycoconjugates Present in Abomasal Epithelium of the Goat) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, ดร.อุไร พงศ์ชัยฤกษ์, ผู้ช่วยศาสตราจารย์, นางสาวผกาวดี พงษ์เกษ, Takao Nishida สื่อสิ่งพิมพ์:pdf AbstractIn the abomasal epithelium of the goat, glycoconjugates have been studied by means of selective histochemical methods. The staining procedures employed were alcian blue (AB) pH 1.0 AB pH 2.5, Periodic acid Schiff (PAS) and AB pH 2.5-PAS. In addition twelve lectins, Triticum vulgaris WGA, Concana valin A, Glycine max SBA, Dolichos biflorus DBA, Arachis hypogea PNA, Solanum tuberosum STL, Datura stramonium DSA, Ulex europeus UEA-I, Bauhinia purpurea BPA, Ricinus communis RCAI, Lotus tetragonolobus LTA, and Limulus polyphemus LPA were applied to detect saccharides residues. According to the results obtained, the nature of glycoconjugates in mucous epithelium was found to change during cell migration from deep to superficial part. The surface mucous cells contained primarily neutral glycoconjugates with vicinal diol groupings and terminal fucose residues. By contrast, the mucous cells in deep pits and in pyloric glands contained acidic sulfated glycoconjugates with terminal sialic acid residues. The ?-D-mannose, ?-D-glucose, ?-D-galactose, N-acetylglucosamine and N-acetylgalactosamine were also found at the pyloric gland cells while they were smaller in amount at the surface mucous cells. The data suggested that changes of epithelial cells were closely correlated with cellular maturation. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Detection of Glycoconjuagates in Chicken Colonic Epithelium with a Specific Horseradish Peroxidase Conjuagated Lectin) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, นายบุญธรรม จงเจริญ, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractChicken colons were stained with a battery of seven horseradish peroxidase-conjugated lectins : Ricnus communis agglutinin-I (RCA-I) peanut agglutinin, wheat germ agglutinin, Ulex europaeus agglutinin, Limax fiavus agglutinin, Dolichos biflorus agglutinin, and Pisum sativum agglutinin. Lectin staining revealed striking differences in the structure of oligosaccharides between intracellular secretory glycoconjugates in goblet cells and in columnar cells. The mucous granules of goblet cells were found to contain glycoconjugates with sialic acid-galactose dimer,D-mannose,D-glucose, N-acety-D-glucosamine and D-galactose residues. In contrast, terminal or internal N-acety-D-glucosamine and terminal galactose-N (1-3)-acety-D-galactosamine disaccharides were detected in supranuclear region of the columner cells. The striated border of the colonic columnar cells were also found to contain glycoconjugates similar in reactivity to those of the goblet cells. The existence of glycoconjugates with various saccharide residues in colonic epithelium of the chicken is reported for the first time in this study. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Detection of Glycoconjugates in Colonic Epithelium of the Goat) ผู้เขียน:นางสาวผกาวดี พงษ์เกษ, Suppalak Romrattanapun, นางมาลีวรรณ เหลี่ยมศิริเจริญ, รองศาสตราจารย์, ดร.ดลดา ศรีใส, อาจารย์, ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe distribution of glycoconjugates in the goat descending colon was studied by means of light microscopic histochemical methods. The staining procedures employed were horseradish peroxidase conjugated lectin, alcian blue (AB) pH 2.5, periodic acid-Schiff (PAS), ABpH 2.5-PAS, high iron diamine (HID), HID-ABpH 2.5. The lectins used in the present study were concanavalin A (ConA), Ricinus communis agglutinin I(RCA-I), Solanum tuberosum agglutinin (STL) and Limulus polyphemus agglutinin(LPA) . The result revealed that descending colonic epithelium of the goat contained acid sulfated glycoconjugates and neutral glycoconjugates with ?-D-mannose, ?-D-glucose, ?-D-galactose, Nacetylglucosamine and sialic acid residues. The quantity of neutral glycoconjugates with ?-D-mannose, ?-D-glucose, ?-D-galactose and N-acetylglucosamine increased from mucosal crypt up to mucosal surface of the epithelium, while sialic acid residues and sulfated glycoconjugates decreased from mucosal crypt to mucosal surface. |
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