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 ผลงานตีพิมพ์ในวารสารวิชาการHistology and Glycoconjugates Histochemistry in the Small and Large Intestinal Epithelium of the Malayan Pangolin, Manis javanicaผู้แต่ง: Dr.Apinun Suprasert, Professor , Mrs.Maleewan Liumsiricharoen, Associate Professor , Ms.Pakawadee Pongket, Dr.Teerasak Prapong, Assistant Professor , Mrs.Apantree Chantong, Mrs.Nathanan Prompa, วารสาร: |
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ผลงานตีพิมพ์ในวารสารวิชาการHistology and Histochemical Distribution of Goblet Cells in the Descending Colonic Epithelium of the Swamp Buffalo (Bubalus bubalis)ผู้แต่ง:Ms.Pakawadee Pongket, Mrs.Maleewan Liumsiricharoen, Associate Professor,  Supalak Romratanapan, Mr.Somchai Pongjunyakul, Associate Professor, Dr.Urai Pongchairerk, Assistant Professor, Dr.Apinun Suprasert, Professor, วารสาร: 
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การประชุมวิชาการจุลกายวิภาคศาสตร์และเนื้อเยื่อเคมีของ goblet cell ในเยื่อบุผิวลำไส้ส่วน descending colon ของกระบือปลัก (Bubalus bubalis) (2009)ผู้แต่ง:Ms.Pakawadee Pongket, Mrs.Maleewan Liumsiricharoen, Associate Professor, Mrs.Suppaluk Romratanapun, Associate Professor , Mr.Somchai Pongjunyakul, Associate Professor, Dr.Urai Pongchairerk, Assistant Professor, Dr.Apinun Suprasert, Professor, การประชุมวิชาการ: |
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histology and Histochemical Distribution of Goblet Cells in the Descending Colonic Epithelium of the Swamp Buffalo(Bubalus bubalis)) สื่อสิ่งพิมพ์:pdf AbstractPredominant glycoconjugates in the descending colon are synthesized and secreted by goblet cells. Changes in the chemical composition of the intestinal mucous affect the functioning of the large intestine. The expression of glycoconjugates in the colonic epithelium of buffaloes was studied using histochemical methods. Paraffin sections 3 ?m thick were stained with the following reagents: 1) haematoxylin and eosin (H&E), 2) alcian blue (AB) pH 2.5, 3) periodic acid –Schiff (PAS), 4) AB pH 2.5 – PAS, 5) high iron diamine (HID), 6) HID–AB pH 2.5, and three lectins, 7) Arachis hypogeae (PNA), 8) Ulex europaeus (UEA-I) and 9) Triticum vulgaris (WGA). Neuraminidase was used before staining with PNA. The histology of the descending colonic epithelium is characterized by an absence of villi; the epithelium have numerous simple columnar crypts. The crypts consist of columnar absorptive cells and goblet cells. The goblet cells in the descending colon of buffaloes contained acid and neutral glycoconjugates, whose expression was increased from the lower crypts to the upper crypts. There were more sulphated glycoconjugates in the goblet cells of lower crypts than in upper crypts. The crypts of the upper areas showed more intense expression of carboxylated glycoconjugates and sialic acid together with D- galactose and N- acetylglucosamine. The expression of ? -L- fucose was found in the goblet cells throughout all crypts. The results indicated that sulfomucin and ? -L- fucose played a lubricating role, while sialic acid and sialoderivatives provided protection against pathogenic infection. |
 ที่มา:มหาวิทยาลัยเกษตรศาสตร์หัวเรื่อง:การศึกษาความสามารถของแบคทีเรียกรดแล็กทิกในการยับยั้งการยึดเกาะเซลล์ลำไส้มนุษย์ของแบคทีเรียที่ก่อโรคอาหารเป็นพิษ |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Characterization of Glycoconjugates Present in Cloacal Epithelium of the Chicken) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, นายบุญธรรม จงเจริญ, รองศาสตราจารย์, Suppaluk Romrattanapun สื่อสิ่งพิมพ์:pdf AbstractThe distribution of glycoconjugates in the chicken cloaca was studied by means of light microscopic histochemical methods. The staining procedures employed were horseradish peroxidase conjugated lectin, Alcian blue (AB) pH 1.0, AB pH 2.5, periodic acid –Schiff (PAS), AB pH 2.5-PAS in combination with enzyme neuraminidase digestion. The lectin used in the present study were peanut agglutinin (PNA), wheat germ agglutinin (WGA), Dolichos biflorus agglutinin (DBA), and Lotus tetragonolobus agglutinin (LTA) The mucosa of both coprodeum and urodeum is covered by simple columnar epithelium in which 2 type of cells are encountered-columnar and goblet cells. In contrast, one layer of only columna cells is found in the mucosa of proctodeum. Tubular glands of proctodeum are commonly provided at submucosal layer. All the goblet cells in coprodeum and urodeum and tubular glands of proctodeum were coloured blue and purple with AB pH 1.0 and AB pH 2.5-PAS, respectively. They were furthermore stained moderately with WGA. Neuraminidase digestion changed the blue coloration with AB pH 2.5 to pale blue. Removal of sialic acid with neuraminidase imparted weak to strong affinity of PNA. Histophysiological significances of glycoconjugates involved in the chicken cloaca were discussed with special reference to the functional activities of the carbohydrates. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Detection of Glycoconjugates in Colonic Epithelium of the Goat) ผู้เขียน:นางสาวผกาวดี พงษ์เกษ, Suppalak Romrattanapun, นางมาลีวรรณ เหลี่ยมศิริเจริญ, รองศาสตราจารย์, ดร.ดลดา ศรีใส, อาจารย์, ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe distribution of glycoconjugates in the goat descending colon was studied by means of light microscopic histochemical methods. The staining procedures employed were horseradish peroxidase conjugated lectin, alcian blue (AB) pH 2.5, periodic acid-Schiff (PAS), ABpH 2.5-PAS, high iron diamine (HID), HID-ABpH 2.5. The lectins used in the present study were concanavalin A (ConA), Ricinus communis agglutinin I(RCA-I), Solanum tuberosum agglutinin (STL) and Limulus polyphemus agglutinin(LPA) . The result revealed that descending colonic epithelium of the goat contained acid sulfated glycoconjugates and neutral glycoconjugates with ?-D-mannose, ?-D-glucose, ?-D-galactose, Nacetylglucosamine and sialic acid residues. The quantity of neutral glycoconjugates with ?-D-mannose, ?-D-glucose, ?-D-galactose and N-acetylglucosamine increased from mucosal crypt up to mucosal surface of the epithelium, while sialic acid residues and sulfated glycoconjugates decreased from mucosal crypt to mucosal surface. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Postnatal cytodifferentation of the columnar and basic cells in the epithelium of the terminal segment of the ram epididymis) ผู้เขียน:Nati Nalnophakoon สื่อสิ่งพิมพ์:pdf AbstractThe postnatal differentiation of cpithelial cells in the terminal segment of the ram epididymis was studied by electron microscopy. At one week the low columnar cells had little cytoplasm with few and primitive organelles. At two weeks differentiation was already obvious, with an increase in the number of cytoplasmic organelles. Precursors of stereocilia were also seen. At four weeks a mature type of ultrastructure was seen in the distal part of the segment, and at six weeks throughout the whole cauda. Spermatozoe were not numerous until at 18 weeks and then still showed an immature fine structure. Basal cells were seen to form from some columnar cells, which first developed hemidesmomes basally. Later, they lost their luminal contact and their cytoplasm accumulated around a laterally displaced nucleus. Invading cells appeared to be lymphocytes that never showed feathers suggesting a transformation to basal cells. The epithelium of the cauda epididymidis [terminal segment] in rams thus acquires a mature structure at an age when the testis still shows solid cell cords only, which are not likely to produce any fluid for transport into the epididymis. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Lectin Histochemical Characterization of Glycoconjugates Present in Abomasal Epithelium of the Goat) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, ดร.อุไร พงศ์ชัยฤกษ์, ผู้ช่วยศาสตราจารย์, นางสาวผกาวดี พงษ์เกษ, Takao Nishida สื่อสิ่งพิมพ์:pdf AbstractIn the abomasal epithelium of the goat, glycoconjugates have been studied by means of selective histochemical methods. The staining procedures employed were alcian blue (AB) pH 1.0 AB pH 2.5, Periodic acid Schiff (PAS) and AB pH 2.5-PAS. In addition twelve lectins, Triticum vulgaris WGA, Concana valin A, Glycine max SBA, Dolichos biflorus DBA, Arachis hypogea PNA, Solanum tuberosum STL, Datura stramonium DSA, Ulex europeus UEA-I, Bauhinia purpurea BPA, Ricinus communis RCAI, Lotus tetragonolobus LTA, and Limulus polyphemus LPA were applied to detect saccharides residues. According to the results obtained, the nature of glycoconjugates in mucous epithelium was found to change during cell migration from deep to superficial part. The surface mucous cells contained primarily neutral glycoconjugates with vicinal diol groupings and terminal fucose residues. By contrast, the mucous cells in deep pits and in pyloric glands contained acidic sulfated glycoconjugates with terminal sialic acid residues. The ?-D-mannose, ?-D-glucose, ?-D-galactose, N-acetylglucosamine and N-acetylgalactosamine were also found at the pyloric gland cells while they were smaller in amount at the surface mucous cells. The data suggested that changes of epithelial cells were closely correlated with cellular maturation. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histochemical Detection of Glycoconjuagates in Chicken Colonic Epithelium with a Specific Horseradish Peroxidase Conjuagated Lectin) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, นายบุญธรรม จงเจริญ, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractChicken colons were stained with a battery of seven horseradish peroxidase-conjugated lectins : Ricnus communis agglutinin-I (RCA-I) peanut agglutinin, wheat germ agglutinin, Ulex europaeus agglutinin, Limax fiavus agglutinin, Dolichos biflorus agglutinin, and Pisum sativum agglutinin. Lectin staining revealed striking differences in the structure of oligosaccharides between intracellular secretory glycoconjugates in goblet cells and in columnar cells. The mucous granules of goblet cells were found to contain glycoconjugates with sialic acid-galactose dimer,D-mannose,D-glucose, N-acety-D-glucosamine and D-galactose residues. In contrast, terminal or internal N-acety-D-glucosamine and terminal galactose-N (1-3)-acety-D-galactosamine disaccharides were detected in supranuclear region of the columner cells. The striated border of the colonic columnar cells were also found to contain glycoconjugates similar in reactivity to those of the goblet cells. The existence of glycoconjugates with various saccharide residues in colonic epithelium of the chicken is reported for the first time in this study. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histology and Glycoconjugates Histochemistry in the Small
and Large Intestinal Epithelium of the Malayan Pangolin,
Manis javanica) ผู้เขียน:ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์, นางมาลีวรรณ เหลี่ยมศิริเจริญ, รองศาสตราจารย์, Pakawadee Pongket,, Teerasak Prapong,, Apantree Doungern, นางธนัตพร พรหมพา สื่อสิ่งพิมพ์:pdf AbstractHistology and glycoconjugates histochemistry in the small and large intestinal epithelium of
the Malayan Pangolin (Manis javanica) were studied by means of histological and histochemical
techniques. The staining procedures employed were Hematoxylin-Eosin (H&E), Alcian Blue (AB) pH
2.5, Periodic Acid-Schiff (PAS), AB pH 2.5-PAS and lectin including penut agglutinin (PNA), Ulex
europeus agglutinin-I (UEA-I), wheat germ agglutinin (WGA) and Maackia amurensis (MAL-II).
The intestinal canal displayed no divivsion between small and large intestine, other than a
continuous enlargement, and there was no cecum. The mucous membrane of the pangolin small intestine
was thrown into high villi and long crypt of Lieberkuhn. While, the mocosa of large intestine lied in a
series of irregular fold, not villi. The mucosa of both intestine was covered by simple columnar epithelium
in which 2 type of cells were encountered columnar and goblet cells. The mucous granule of goblet cells
in small intestine were found to contain primarily neutral glycoconjugates with galactose and sialic
acid residues. On the contrary, the mucous epithelium of large bowel involved predominantly acid
sulfated glycoconjugates with N-acetylglucosamine, fucose and sialic acid residues. Furthermore, the
nature of glycoconjugates in mucous epithelium of both intestine was found to change during the upward
migration from deep to superficial part. |
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