ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Evaluation of the Appropriate Diagnostic Tools for Intra-Mammary Infection in Lactating Dairy Goats)

ผู้เขียน:Jitkamol Thanasak, Nareerat Sangkachai, Kulanan Imsawang, Surasak Jittakhot

สื่อสิ่งพิมพ์:pdf

Abstract

Sixty milk samples of healthy, lactating dairy goats were collected by an aseptic, hand milking technique. All samples were analyzed using milk quality tests: bacterial isolation (Bac), total plate count (TPC), dye reduction test (DRT) and clot-on-boiling test (COB), as well as screening tests: the Californian mastitis test (CMT) and electrical resistance (ER), and somatic cell count (SCC) and percentage of milk composition including fat, protein, lactose and solid-not-fat (SNF). According to 34 samples for intra-mammary infection (IMI) and 26 samples for non-IMI, the results showed that Bac and TPC were the most reliable techniques for the determination of IMI with strong correlations. For an instantaneous milk quality test, CMT reaction was probably more reliable as there was a statistical significance between bacteriologically positive and negative samples (P = 0.06). Moreover, CMT showed a correlation with TPC, Bac, DRT, ER, ER ref, SCC, milk protein, and milk lactose (P ? 0.05). Based on the range in ER, TPC showed a statistically significant difference between the ER level being less than 350 units and higher than 400 units (P ? 0.05). Except for CMT reactions, SCC did not correlate to other measurements and did not indicate the IMI status of goats. These findings indicated that there was a need for future improvement in the diagnostic tools for IMI in goats.

Article Info

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 043, Issue 2, Apr 09 - Jun 09, Page 288 - 296 |  PDF |  Page

ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Estrus Synchronization Using Intravaginal Medroxyprogesterone Acetate (MAP), MAP Plus Prostaglandin F2?, Controlled Internal Drug Release (CIDR) or CIDR Plus Prostaglandin F

ผู้เขียน:Jatuporn Kajaysri, Chanathip Thammakarn

สื่อสิ่งพิมพ์:pdf

Abstract

The objective of this study was to compare the effi ciency of medroxyprogesterone acetate (MAP) in the form of impregnated intravaginal sponges with or without Prostaglandin F2? (PGF2?) and natural progesterone impregnated controlled internal drug release (CIDR) devices with or without PGF2? in dairy Saanen goats in Thailand. Seventy-fi ve Saanen does were divided into fi ve equal groups. The sixty does in Groups 1 to 4 in Experiment 1 were assigned for estrus synchronization using the following intravaginal progestagen techniques: sponges impregnated with MAP in Group 1 (n=15), sponges with MAP and intramuscular injection of 125 g PGF2? in Group 2 (n=15), progesterone CIDR devices in Group 3 (n=15) and progesterone CIDR devices and intramuscular injection of 125 g PGF2? in Group 4 (n=15). Intravaginal progestagens were administered for 13 d and the PGF2? was injected on day 13. Estrus responses of does in Groups 1 to 4 were detected from 12 to 60 hr after progestagen withdrawal. In the remaining 15 does of Experiment 2 in Group 5, estrus was synchronized by intravaginal progestagen with MAP for 13 d. Estrus responses of does in Group 5 were detected at 24 and 48 hr after progestagen withdrawal. After estrus synchronization, the estrus does in Groups 1 to 5 were artifi cially inseminated with frozen semen once within 48–50 hr after progestagen withdrawal. However, for does in estrus in Group 5, the vaginal mucous accumulations were fl ushed with saline solution before artifi cial insemination (AI). Pregnancy was detected by ultrasonography on day 60 after AI. The result of Experiment 1 was estrus responses in all does in Groups 1, 2 and 4 but in only 10 does in Group 3. The mean estrus onset times of Groups 1 and 2 were not signifi cantly different (21.0 ? 1.2 and 21.5 ? 1.3 hr, respectively) but were signifi cantly different from those of Groups 3 (18.5 ? 1.9 hours) and 4 (18.6 ? 2.0 hr) (P < 0.05). The mean estrus durations of Groups 1 to 4 were not signifi cantly different. Pregnancy ultrasonography revealed no difference in the pregnancy rates among Groups 1 to 4. Experiment 2 resulted in estrus responses in all does in Group 5. Comparison of the pregnancy rates of Groups 1 and 5 showed that Group 5 had a trend of a higher pregnancy rate than Group 1. It can be concluded that the MAP treatment with or without PGF2? had equal effi cacy to the CIDR-with-PGF2? treatment, and that the MAP treatment combined with vaginal mucous fl ushing could possibly increase the conception rate.

Article Info

Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 046, Issue 1, Jan 12 - Feb 12, Page 71 - 79 |  PDF |  Page