Search Result of "Chongkolnee Weerasathakul"

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effect of Cryoprotectants on Cryopreservation of Sugarcane Cells )

ผู้เขียน:ImgSonthichai Chanprame, Imgดร.เรวัต เลิศฤทัยโยธิน, รองศาสตราจารย์, ImgChongkolnee Weerasathakul



Cell suspension of sugarcane cultivar ROC3 treated with different concentration and combination of cryoprotectant were cryopreserved. Before cryopreservation, temperature of cell suspension was reduced from room temperature to O?C/ min. and from O?C to -40?C at the rate of 0.5?C/min.. Then, cell suspension were storaged in liquid nitrogen for 30 minutes. After storage, sugarcane cells were recultured again on solid media. Sugarcane cells treated with 15% dimethysulphoxide (DMSO) and 1.OM sucrose showed the best regrowth among the cryoprotectants studied.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 027, Issue 5, Jan 93 - Dec 93, Page 1 - 3 |  PDF |  Page