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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Species Identification of 3 Hypsibarbus spp. (Pisces: Cyprinidae) Using PCR–RFLP of Cytochrome b Gene) ผู้เขียน: ดร.พัฒนี จันทรโรทัย, รองศาสตราจารย์ ,  สุรภพ สุทธิวิเศษ, Wongpathom Kamonrat, สุรินทร์ ปิยะโชคณากุล, Chavalit Vidthayanon สื่อสิ่งพิมพ์:pdf AbstractPolymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP) was used to identify 3 closely related Hypsibarbus spp: Hypsibarbus wetmorei, H. vernayi, and H. malcolmi. Mitochondrial cytochrome b gene (993 bp) in 3 Hypsibarbus spp. showed single PCR– product. The sequencing results of PCR–products in 3 Hypsibarbus spp. showed very low interspecific variation. However it could be used to discriminate these species by RFLP analysis. The combination of 2 restriction enzymes; Bsp143I and BcuI were used to identify 3 Hypsibarbus spp. Bsp143I could discriminate H. vernayi from H. wetmorei and H. malcolmi, by generating 3 fragments (535 bp, 234 bp and 224 bp) in H. vernayi whereas 2 fragments of 769 bp and 224 bp in H. wetmorei and H. malcolmi. Thereafter, BcuI was effectively discriminated H. wetmorei from H. malcolmi by generating 3 fragments (591 bp, 288 bp and 114 bp) in H. malcolmi and uncut fragment in H. wetmorei. There were intraspecific restriction polymorphism in H. vernayi using BcuI which generated 2 patterns; an uncut fragment and 2 fragment of approximately 700 bp and 300 bp. Thus, PCR–RFLP technique could be used to identify 3 closely related Hypsibarbus spp. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Community Structure of Aquatic Plants in Bung Khong Long, Nongkhai Province, A Ramsar Site of Thailand) ผู้เขียน:ไชยา อุดมศรี, นางสาวศิรประภา เปรมเจริญ, ผู้ช่วยศาสตราจารย์ , Chitraporn Thawatphan, Chavalit Vidthayanon, ดร.สรัญญา วัชโรทัย, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe community structure of aquatic plant in Bung Khong Long, a Ramsar site of Thailand was conducted during July 2001 to April 2002 to study the species diversity, density and distribution. Six 1 m2 quadrates were randomly sampled in five different plant communities during three seasons. A total of 75 species, 62 genera, and 38 families was found with an average biomass of 296.50 g/m2. The dominant species were Utricularia aurea, Hydrilla verticillata and Eleocharis dulcis var. dulcis with the Important Value Index (IVI) 84.66, 54.30 and 31.09%, respectively. This area had a high species diversity with H value 3.24 (Shannon-Weaver diversity index). The aquatic plants grew profusely in the rainy season especially the submerged and the free floating groups whereas the emerged and marginal groups grew profusely in summer. In the northern part of the reservoir, water surface was evenly covered by islets and free floating plants whereas the central part was the habitat of emerged plants such as Nelumbo nucifera and Nymphaea lotus. In contrast, the southern part was open water area and deeper. It was the habitat of submerged plants such as Najas sp.1 and Chara sp.1 without islets and free floating plants. A marginal plant, Caldesia sp.1 was found in the northern part. In addition, at least 34 species of aquatic plants were collected by local people for using as food, fodder and other purposes. |
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