Search Result of "Chainarong Rattankkreetakul"

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Isolation of Acaricidal Substances against Tropical Cattle Ticks from Sugar Apple Seeds)

ผู้เขียน:Imgดร.ณรงค์ จึงสมานญาติ, รองศาสตราจารย์, ImgSugunya Mahatheeranont, ImgChainarong Rattankkreetakul, Imgดร.สุรัตน์วดี จิวะจินดา, Imgนายวีระพล จันทร์สวรรค์, รองศาสตราจารย์



Isolation of acaricidal active substances from sugar apple seeds (Annona squamosa L.) against tropical cattle ticks (Boophilus microplus) was performed. Two active compounds were found in ethyl acetate part of ethanol extracted crude. The compound I (R 0.59) was isolated from twice chromatographed fractions of 60-100% ethyl acetate/toluene (F4-6) and 10, 20% ethanol/ethyl acetate (F7-8), and of 10% ethanol/ethyl acetate (FF 7) by preparative TLC (developed with hexane:acetone; 3:2) and preparative HPLC (reverse phase C subscript 18 column, 85% MeOH in water, 210 nm UV detecter). The compound II (R 0.31) was isolated from triple chromatographed of 80% ethyl acetate/toluene (F5), 45-60% acetone/hexane (F A), and of 40-50% ethyl acetate/toluene (FF B) respectively by preparative TLC (developed with hexane:acetone, 3:2). The compound I and II displayed a highest mass in EI mass spectrum at 623 and 432 mass unit, respectively. The fragmentation pattern of compound I are similar to those known substance; squamocin and acetogenin. The protron NMR spectrum of both compounds displayed the similarly pattern of triterpenoid.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 026, Issue 5, Jan 92 - Dec 92, Page 41 - 45 |  PDF |  Page