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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Ca2+ATPase, Carbonic Anhydrase and Alkaline Phosphatase Activities in Red Sternum Mud Crabs (Scylla serrata)) ผู้เขียน: Thanaset Tongsaikling,  ดร.จินตนา สและน้อย, รองศาสตราจารย์ , ดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractRed sternum is a poorly understood symptom affecting mud crab aquaculture in Thailand. This work presents the results of enzymatic activities in mud crab exhibiting the red sternum symptom. The enzymatic activities of Ca2+ATPase, carbonic anhydrase and alkaline phosphatase were analyzed in five tissues (muscle, gill, hepatopancreas, integument and hemolymph) from symptomatic and asymptomatic mud crabs. The main features of the symptom include reddish colored stripes on the abdominal thorax, sternum and chelae, swollen hepatopancreas, milky hemolymph and opaque and whitish muscle. The most distinctive change could be observed in the hemolymph which enabled classification of its severity into three groups—group I: translucent brown hemolymph, group II: cloudy orange hemolymph and group III: milky unclotted hemolymph. Ca2+ATPase levels were highest in the muscle of asymptomatic crabs. This enzyme activity tended to increase in all tissues of symptomatic crabs in groups I and II, especially in the gill of group II where it was fivefold higher than in asymptomatic crabs. Carbonic anhydrase and alkaline phosphatase values were highest in the hepatopancreas of asymptomatic crabs. Alkaline phosphatase decreased in all tissues of the three groups of symptomatic crabs. Carbonic anhydrase levels were highly variable with a decreasing trend in most tissues of groups I and II. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Chitinase and Carbonic Anhydrase Activities during Molting Cycle
of Mud Crab (Scylla serrata Forskal 1775)) ผู้เขียน:ดร.จินตนา สและน้อย, รองศาสตราจารย์, ดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์, ดร.อรุณี อิงคากุล, ผู้ช่วยศาสตราจารย์ , ประทักษ์ ตาบทิพย์วรรณ, นางอมรา ทองปาน, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractOptimum conditions for the activities of chitinase and carbonic anhydrase in gill, integument and
haemolymph of mud crab (Scylla serrata Forskal 1775) were determined. The maximum activity of
chitinase in all 3 types of tissue was observed at pH 11 and 40?C. However, optimum conditions for
carbonic anhydrase in gill, integument and haemolymph were at pH 7.6 at 50?C, pH 7.2 at 50?C and pH
7.2 at 60?C, respectively. Both chitinase and carbonic anhydrase activities varied during various stages
of molting. For chitinase, activity in gill at 2-day premolt was significantly lower (P<0.01) than that at
6 hr to 5-day postmolt but not significantly (P<0.01) different from that at intermolt. Maximum chitinase
activity in integument observed at 24 hr postmolt was significantly higher (P<0.01) than that at intermolt
and early premolt. Chitinase activity in haemolymph was quite stable during premolt and postmolt but
significantly lower than that at intermolt (P<0.01). Observation of carbonic anhydrase activities in gill
and integument revealed significantly higher activity after molting than during intermolt and premolt
stages. Maximum activity of carbonic anhydrase in gill and integument was evident at 6 hr and 5-day
postmolt, respectively. For haemolymph, the level of carbonic anhydrase activities during various stages
of molting cycle were highly significantly different (P<0.01). The results indicated important roles of
chitinase and carbonic anhydrase at every stage in molting cycle of mud crab. |
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Researcherดร. จินตนา สและน้อย, รองศาสตราจารย์ที่ทำงาน:ภาควิชาวิทยาศาสตร์ทางทะเล คณะประมง บางเขน สาขาที่สนใจ:สารสีในแพลงค์ตอนพืช, กรดไขมันสารสี, เอนไซม์ในสัตว์ไม่มีกระดูกสันหลัง, เทคโนโลยีชีวภาพทางทะเล Resume |