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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Na+, K+ and Ca2+ Accumulation on the Expression of Ca2+-ATPase Gene in Rice KDML 105) ผู้เขียน: วรรณรดา สุราช,  ดร.มิ่งขวัญ มิ่งเมือง, รองศาสตราจารย์ , นางอมรา ทองปาน, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractHaving been exposed to 150 mM NaCl supplemented in the growth medium, the roots of KDML105 started to accumulate Na+ in three hours but the leaves showed the amount of Na+ change after one day. Addition of either 0.4 mM or 10 mM of CaCl2 to the 150 mM NaCl supplemented medium, on the other hand, caused a small drop in Na+ accumulation in the leaves and roots on day 8. K+, however, significantly decreased in both leaves and roots as the result of NaCl in the medium. As for Ca2+ deposition in KDML105, NaCl supplemented alone in the medium had little effect but the combined NaCl and CaCl2 caused the rise of Ca2+ in both leaves and roots. The expression of Ca2+-ATPase gene in the leaves of KDML105 seemed to be directly activated by the exposure to Na+. The combined effect of Na+ and Ca2+ also showed the drastic change in Ca2+- ATPase expression, and rice, therefore, try to maintain Ca2+ homeostasis by producing more Ca2+- ATPase mRNA especially in the leaves. Since the roots are not the main accumulation site of Na+, the effect of NaCl alone on the Ca2+-ATPase gene in the roots was not as distinctively seen. However, the addition of 10 mM CaCl2 caused a large increase of Ca2+-ATPase gene expression in the roots, while both Na+ and Ca2+ accumulation seemed to affect more to the gene expression in the leaves. Key words: Na+, K+, Ca2+, rice KDML105, Ca2+-ATPase gene, Ca2+-ATPase mRNA |
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