Search Result of "C. Chetanachitra"

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Chemical, Biochemical and Physical Treatments on the Kinetics and on the Role of Some Endogenous Enzymes Action of Baker’s Yeast Lysis for Food-Grade Yeast Extrac

ผู้เขียน:ImgS. Boonraeng, ImgP. Foo-trakul, ImgW. Kanlayakrit, ImgC. Chetanachitra

สื่อสิ่งพิมพ์:pdf

Abstract

The effects of chemical, biochemical and physical treatments on lysis of baker’s yeast cells were studied at pH 5.2 and temperature 54 ?C. An unstructured kinetic model of first order was developed to understand the mechanism of yeast lysis. The autolysis rate constant (k), yield factor for protein (?P), total carbohydrate (?C) and autolysate (?A) were sensitive to disruption methods. Since there were endogenous enzymes in the yeast cells such as proteinases, glucanases and carboxypeptidases, these enzymes were responsible for synergistic of cell disruption and their activity profiles were affected by different treating methods. From the determined results of amino acids composition in yeast autolysate, there were differences in amino acid content. The biochemical treated sample using 0.1% papain gave the highest total amino acid content among all analysed samples.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 034, Issue 2, Apr 00 - Jun 00, Page 270 - 278 |  PDF |  Page 

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ที่มา:วิทยาสารเกษตรศาสตร์ สาขา วิทยาศาสตร์

หัวเรื่อง:การปรับสภาพที่เหมาะสมด้วยวิธีทางเคมีชีวภาพและกายภาพสำหรับการย่อยสลายยีสต์ขนมปังเพื่อผลิตยีสต์สกัด

ผู้เขียน:Imgนายประศาสตร์ ฟูตระกูล, ผู้ช่วยศาสตราจารย์, ImgSupot BOONRAENG, ImgWeerasit Kanlayakrit, ImgCharan Chetanachitra

สื่อสิ่งพิมพ์:pdf

Abstract

The effects of chemical, biochemical and physical treatments on baker?s yeast lysis were studied. The standard conditions for autolysis were pH 5.2, temperature 54?C and yeast concentration 13% dry wt. The optimum chemical treatment obtained by using 5% v/v ethanol and 5% w/v NaCl. The optimum biochemical and physical treatments were the use of 0.1%w/w of papain and 8,000 psi of homogenization respectively. The combination treatment of 5% ethanol and 5% NaCl exhibited highest protein content (61.90%).The physical treated sample obtained 42.45% of total carbohydrate yield and biochemical treated sample obtained 46.53% of autolysate. The chemical analysis of the samples showed the best result in the biochemical treated sample as (g/100g dry wt): 57.50 of total protein, 8.22 of amino-nitrogen, 24.27 of carbohydrate, 7.86 of nucleic acid and 10.72 of ash contents. This treatment showed the feasibility of producing yeast extract, yeast autolysate at pilot plant or industrial scale.

Article Info
Agriculture and Natural Resources -- formerly Kasetsart Journal (Natural Science), Volume 032, Issue 4, Oct 98 - Dec 98, Page 441 - 451 |  PDF |  Page