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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Effects of Culture Media and Growing Media on Kluai Bep) ผู้เขียน: อัญชุลี ชินสุข,  นางเบญจมาศ ศิลาย้อย, ศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractIn vitro, culture of Kluai Bep was conducted in MS media supplemented with BA at 4 levels of concentration of 1,3,5 and 7 ppm. Subcultures were prepared every month. At high concentration of BA, more proliferation and shoot were obtained. Average proliferation time of BA cultured, on media of 1,3,5 and 7 ppm, were found to be 45, 41, 36 and 35 days respectively. It was also noticed that the numbers of shoot, increasing at the seventh month in 7 and 5 ppm BA, were 5.71 and 5.57, while those of 3 and 1 ppm BA 4.29 and 3.86 shoots/plant respectively. Shoots of 5 and 7 ppm BA were not significantly different from those of 1 and 3 ppm BA. All the plantlets were transferred to 1/2 MS without hormone for rooting. The survival rate of K. Bep after transplanting was 81.71 per cent. The horticultural characteristics such as height, size of plant, size of pseudostem and leaf were recorded after growing in 4 and 6 inches pots with different growing media. K. Bep growing in 4 inches pots with 2 parts soil mix : 2 parts of sand and 1 part of coir dust showed the best development on the ninth month according to the above character . Morphology of the 7th generation of mature K. Bep were solid mutant of K.Khai. |
หัวเรื่อง:การปรับปรุงพันธุ์ข้าวโดยการเพาะเลี้ยงอับเรณูข้าวลูกผสม ระหว่าง เลมอนท์และขาวดอกมะลิ 105 ผู้เขียน:P. Pongtongkam, R. Lertvichai, P. Ratisoontorn, B. Silayoi, S. Apisittivanich, S. Peyachoknagul, S. Supittitada, L. Ngernsiri, K. Klakhaeng สื่อสิ่งพิมพ์:pdf AbstractThe panicles of the F1 hybrid rice (Lemont/Khao Dawk mali 105) of which the distance between the auricles of the flag leaf and the subtending leaf was 6-12 cm were selected for anther culture. These spikelets had anthers bearing the mid-uninucleate microspores. High percentage of callus formation was obtained when anthers were cultured on the N6 medium supplemented with 500 mg/l casein hydrolysate, 2 mg/l NAA, 1 mg/1 kinetin and 50 g/l maltose. The calli could regenerate into plantlets at high frequency when cultured on the MS medium containing 15% coconut water, 300 mg/l casein hydrolysate, 20 g/l sucrose, 2 mg/lBAP and 0.2 mg/l NAA. About forty percent and forty-two percent of double haploid plants were obtained when the regenerants were transferred on the MS medium containing 1 g/l yeast extract, 15% coconut water, 3 mg/ 1 BAP and 0.01% colchicines for 3 and 5 days, respectively. |
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หัวเรื่อง:จำนวนและรูปร่างของโครโมโซมของกล้วยบางชนิดในประเทศไทย ผู้เขียน:นางเบญจมาศ ศิลาย้อย, ศาสตราจารย์, Prawat Sompen สื่อสิ่งพิมพ์:pdf AbstractThe chromosome number of thirty accessions of bananas, both wild and cultivated ones were investigated by squashing root tip technique. There were 11 diploids (2n = 22) namely: Kluai Pa number 1, Kluai Pa number 3, Kluai Ang Kang, Kluai Khae, Kluai Khai, Kluai Tani, Kluai Khai Boran, Kluai Thong Khi Meaw, Kluai Pa Nunber 22, Kluai Lai and Kluai Mak. The triploids (2n = 33) were Kluai Khrao, Kluai Neu Mu Nang, Kluai Nam Kap Dum, Kluai Kung Khieo, Kluai Nam, Kluai Tip, Kluia Leb Chang Kut, Kluai Tip Khum, Kluai Khom Bao, Kluai Khom Nak, Kluai Namwa Luang, Kluai Kugn, Kluai Klong Chang, Kluai Phama Haek Kuk, Kluai Nang Klai, Kluai Hom Tia, Kluai Namwa Khom and Kluai Khai Bong. Only Kluai Teparot was tetraploid (2n=44) The karyotype of those chromosomes were studied by measuring the length of long and short arms and grouped them into metacentric, submetacentric, subtelocentric and telocentric. The lengths varied from 1.22 to 3.93 microns. The chromosome of ‘acuminata’ raletives were metacentric and submetacentric such as : Kluai Pa number 1, Kluai Pa number 3, Kluai Khae, Kluia Khai, Kluia Khai Boran, Kluia Thong Khi Meaw, Kluai Lai, Kluai Mak, Kluai Khrao, Kluai Kung Khieo, Kluai Kung, Kluai Klong Chang and Kluai Khai Bong, the rests were hybrids of Musa acunimata Colla. And Musa ballbisiana Colla. The karyotypes were metacentric, submetacentric and subtelocentric. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Meiotic Behavior in Microsporocytes of Some Bananas in Thailand) ผู้เขียน:เทิดศักดิ์ โทณลักษณ์, นางเบญจมาศ ศิลาย้อย, ศาสตราจารย์, ดร.ยิ่งยง ไพสุขศานติวัฒนา, รองศาสตราจารย์ , นายประดิษฐ์ พงศ์ทองคำ, ศาสตราจารย์เกียรติคุณ สื่อสิ่งพิมพ์:pdf AbstractMeiotic behavior was investigated in the microsporocytes of six wild bananas with AA and BB genomes and 46 cultivated bananas with AA, AAA, BBB, AAB and ABB genomes. All banana material was collected from the Pak Chong Research Station, Kasetsart University, Nakhon Ratchasima, Thailand. Most of the wild bananas showed normal synapses with a high chromosome pairing frequency of 11 bivalents per pollen mother cell (PMC). The cultivated bananas exhibited many variations in chromosome association at metaphase-I. Cultivars with the AA genome had more univalents than those of wild species with the same AA genome. Univalent, bivalent and trivalent occurrences were also common in all triploid cultivated bananas, but quadrivalents were very rare. The univalents were high in the AAB and ABB genomes, whereas trivalency was high in the AAA and BBB genomes. Micronuclei were observed in the tetrad of all cultivated bananas, but were very low in wild bananas. Many types of tetrad were shown in different orientation patterns at metaphase-II. The occurrence of the parallel disposition pattern was high, but the perpendicular and lineal disposition patterns were not common in any of the bananas. |