 |
|
|
|
|
|
|
 |
|
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : The Effect of Peptidoglycan on Immune Response in Black Tiger Shrimp (Penaeus monodon Fabricius)) ผู้เขียน: ดร.วัชริยา ภูรีวิโรจน์กุล, รองศาสตราจารย์ , ดร.นนทวิทย์ อารีย์ชน, รองศาสตราจารย์ , ดร.ประพันธ์ศักดิ์ ศีรษะภูมิ, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractThe effect of peptidoglycan on immune response in Black Tiger Shrimp (Penaeus monodon Fabricius) was conducted under laboratory conditions. P. monodon (13?2 g) were fed for 7 days with diets containing graded levels of PG (0, 0.06, 0.12 and 0.18 g/kg feed). The immune responses were measured by phenoloxidase, superoxide anion, bactericidal activity, clearance ability and resistance against Vibrio harveyi. The results showed that shrimp which fed on diets containing PG 0.18 g/kg had these immune parameters at significantly higher (P<0.05) than PG 0.06, 0.12 g/kg and control. The study for optimum time of using PG 0.18 g/kg was determined with P. monodon (13?2 g) for 10 weeks. In weeks 1-4, shrimps were fed with the diet containing PG at 0.18 g/kg at 0, 3, 5 and 7 days/week. In weeks 5-8, shrimps were fed with normal feed and in weeks 9-10, shrimps were fed with same concentration and frequency as weeks 1-4. The results showed that optimum time for use of PG 0.18 g/kg was 5 days/week. This application could increase total hemocytes, phenoloxidase, superoxide anion and bactericidal activity which were significantly different (P<0.05) from 3 days/week and control but not significantly different (P>0.05) from 7 days/week. In conclusion, oral administration of PG at 0.18 g/kg diet for 1 month (5 days/week), normal feed for 1 month and PG 0.18 g/kg diet for another month (5 days/week) could effectively enhance the immune system of P. monodon. |
|
|
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Immune Response of Walking Catfish (Clarias macrocephalus Gunther) to Vaccination by Injection, Immersion and Oral Administration) ผู้เขียน:ดร.นนทวิทย์ อารีย์ชน, รองศาสตราจารย์,  Nilubol Kitancharoen, Kamonporn Tonguthai สื่อสิ่งพิมพ์:pdf AbstractWalking catfish (Clarias macrocephalus Gunther) were immunized with vaccine prepared from formalin-killed Aeromonas hydrophila by injection, immersion and oral administration. Antibody titer in the serum and disease protection upon challenge with virulent A. hydrophila were investigated. Walking catfish intraperitoneally injected with vaccine mixed with Freund’s complete adjuvant had the average serum antibody titer of 100.00 and 1105.30 for the primary and secondary immune response while walking catfish vaccinated by intraperitoneal injection with regular vaccine, hyperosmotic immersion and direct immersion had the average titer of 92.20, 444.60; 76.10, 122.60 and 33.75, 61.20 respectively. Injection induced the best protection against A. hydrophila with no mortality while direct and hyperosmotic immersion could protect 60.5 and 77.20 percent of vaccinated fish respectively. Vaccination by oral administration did not show good potency in terms of generating serum antibody titer but still produced better disease protection than unvaccinated fish. |
|
|
|
|
|
|
|