 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Morphological Changes of Apoptosis in the Human Cholangiocarcinoma Cell Line, HuCCA-1, Induced by Green Pit Viper Venom) ผู้เขียน: ดร.อุไร พงศ์ชัยฤกษ์, ผู้ช่วยศาสตราจารย์ , ดร.ศิริรักษ์ จันทครุ, ผู้ช่วยศาสตราจารย์ , นางสาวผกาวดี พงษ์เกษ , นางกฤษณา แสงประไพทิพย์ , Apantree Doungern สื่อสิ่งพิมพ์:pdf AbstractThe venom of the green pit viper (Trimeresurus purpureomaculatus) was used to study the effect on bile duct epithelial cancer cell damage, using the human cholangiocarcinoma cell line, HuCCA-1, as a model. After cell treatment by various concentrations of snake venom at different times, alteration of the cellular structure was observed, especially in the apoptosis of cells. Under a light microscope, many patterns of cellular changes were obvious following treatment with 40 ?g/ml snake venom for six h, while the structure of untreated cells was still unchanged. The number of altered cells was significantly higher than in the control (p<0.05). A transmission electron micrograph showed that venom-treated cells had the characteristic of apoptosis to varying degrees. The cells presented plasma membrane blebs, an increased number of cytoplasmic vacuoles, enlargement or deterioration of organelles, reduced nuclear size, irregular nuclear membrane and clumping of nuclear chromatin. Though snake venom in the Viperidae family causes hemorrhagic symptoms by the function of several components, including phospholipase A2, it was hypothesized that the venom of the green pit viper shared structural similarity with Lys49 phospholipase A2, which lacked a hemolytic activity, but showed an ability to induce cancer cell apoptosis. |
 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Histology and Glycoconjugates Histochemistry in the Small
and Large Intestinal Epithelium of the Malayan Pangolin,
Manis javanica) ผู้เขียน: ดร.อภินันท์ สุประเสริฐ, ศาสตราจารย์ , นางมาลีวรรณ เหลี่ยมศิริเจริญ, รองศาสตราจารย์ , Pakawadee Pongket,, Teerasak Prapong,, Apantree Doungern, นางธนัตพร พรหมพา สื่อสิ่งพิมพ์:pdf AbstractHistology and glycoconjugates histochemistry in the small and large intestinal epithelium of
the Malayan Pangolin (Manis javanica) were studied by means of histological and histochemical
techniques. The staining procedures employed were Hematoxylin-Eosin (H&E), Alcian Blue (AB) pH
2.5, Periodic Acid-Schiff (PAS), AB pH 2.5-PAS and lectin including penut agglutinin (PNA), Ulex
europeus agglutinin-I (UEA-I), wheat germ agglutinin (WGA) and Maackia amurensis (MAL-II).
The intestinal canal displayed no divivsion between small and large intestine, other than a
continuous enlargement, and there was no cecum. The mucous membrane of the pangolin small intestine
was thrown into high villi and long crypt of Lieberkuhn. While, the mocosa of large intestine lied in a
series of irregular fold, not villi. The mucosa of both intestine was covered by simple columnar epithelium
in which 2 type of cells were encountered columnar and goblet cells. The mucous granule of goblet cells
in small intestine were found to contain primarily neutral glycoconjugates with galactose and sialic
acid residues. On the contrary, the mucous epithelium of large bowel involved predominantly acid
sulfated glycoconjugates with N-acetylglucosamine, fucose and sialic acid residues. Furthermore, the
nature of glycoconjugates in mucous epithelium of both intestine was found to change during the upward
migration from deep to superficial part. |