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 ผลงานตีพิมพ์ในวารสารวิชาการbeta-Galactosidase from Lactobacillus pentosus: Purification, characterization and formation of galacto-oligosaccharidesผู้แต่ง: Maischberger, T, Leitner, E,  Dr.Sunee Nitisinprasert, Associate Professor , Juajun, O, Yamabhai, M, Nguyen, TH, Haltrich, D, วารสาร: 
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ผลงานตีพิมพ์ในวารสารวิชาการ?-galactosidase from Lactobacillus pentosus: Purification, characterization and formation of galacto-oligosaccharidesผู้แต่ง:Maischberger, T., Leitner, E., Dr.Sunee Nitisinprasert, Associate Professor, Juajun, O., Yamabhai, M., Nguyen, T.-H., Haltrich, D., วารสาร: 
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 หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Characterization of a Crude Thermostable ?-galactosidase by the Bacterium PD1 Isolated from the Pong Dueat Hot Spring) ผู้เขียน:Phimchanok Nakkharat, Arkom Tesnum, Arkira Maethawarakorn, Dietmar Haltrich, นางจิรกานต์ เมืองนาโพธิ์ สื่อสิ่งพิมพ์:pdf Abstract?-Galactosidase is an enzyme that hydrolyses lactose to glucose and galactose. The present study focused on the characterization of ?-galactosidase from a thermophilic microoganism isolated from the Pong Dueat hot spring, Chaingmai Province, Thailand. o-Nitrophenyl-L-D-galactopyranoside (oNPG) was used as a substrate to determine the ?-galactosidase activity from a strain PD1, which was found to be the active strain producing the highest ?-galactosidase activity. The optimum pH and temperature for enzyme activity were 6.5 and 55?C, respectively. The enzyme was stable at pH range of 6 to 10. The half-life of enzyme at 80, 70, 60, 55, 50 and 45?C were 0.5, 2, 31, 62, 87 and 435 h, respectively. The Km and Vmax values for the synthetic substrate oNPG were 11 mM and 1.1?10-2 mmolL-1min-1, respectively. |
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หัวเรื่อง:การผลิตและการใช้ไฮโตรไลซ์เวย์ ผู้เขียน:ดร.สมจิต สุรพัฒน์ , Sirisak Sarasophon, นางปรียา วิบูลย์เศรษฐ์, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractTemperature control by computer grogram which was designed to control the relay with water pumping comparing with no water pumping, (av. 37.05?0.35?C and 36.98?0.25?C) was better than thermostat control (35.09?0.6?C) when set at 37?C. It gave more accurate and reliable control system. The hydrolysis of lactose by beta-galactosidase showed that the specific activities of varied enzyme concentrations (0.002, 0.006 and 0.010 mg/ml) at 37?C pH 6.8 were not significantly different. Specific activity of enzyme was higher at 135 min (28.92 ?mol/min/mg protein) than at 340 min (17.37 ?mol/min/mg protein) hydrolysis. Organoleptic tests of fermented whey resulted that the taste panelists preferred prducts with total solid contents of 15% and 20% to 10% (p>0.05). Moreover, the taste panel preferred the hydrolyzed fermented whey to the unhydrolyzed sample. |
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ผลงานตีพิมพ์ในวารสารวิชาการวิธีการศึกษาสำหรับกระบวนการการดื้อต่อยาเคมีบำบัดที่ทำลายสารพันธุกรรม ในห้องปฏบัติการ (2016)ผู้แต่ง:Pasarat Khongkow, Anna W. Middleton, , Jocelyn P.-M. Wong, Navrohit K. Kandola, Dr.MESAYAMAS KONGSEMA, Lecturer , Gabriela Nestal de Moraes, Ana R. Gomes, Prof Eric LAM, วารสาร: |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Sorghum 2-Dimensional Proteome Profiles and Analysis of HSP70 Expression Under Salinity Stress) ผู้เขียน:Bongani K. Ndimba, Ludivine A. Thomas, Rudo Ngara สื่อสิ่งพิมพ์:pdf AbstractSorghum (Sorghum bicolor), a drought-tolerant cereal, is the second most important grain crop in Africa after maize (Zea mays). In this study, sorghum proteomes from whole plant organs (leaves, sheaths and roots), as well as cell suspension culture systems were resolved successfully using gelbased proteomics tools. Total soluble proteins (TSP) were extracted from leaves, sheaths and roots, as well as cultured cells. These proteomes were resolved via two-dimensional polyacrylamide gel electrophoresis (2D-PAGE). Proteins were visualized by Coomassie Brilliant Blue (CBB) and identified by MALDI-TOF mass spectrometry (MS). Five different proteomes were studied: total soluble proteome and secretome from cell suspension culture, as well as leaf, sheath and root proteomes from whole plants. Unique protein expression profiles were observed for each of the five proteomes, suggesting specialization of the proteins. Western blot analysis indicated that, as expected, HSP70 protein expression was induced in the roots of the experimental material following salt stress treatments. Cell suspension cultures provide a special research material, particularly for studies related to secreted proteins. The expected secretion of an alpha-galactosidase was also observed. On completion of spot mapping and identification, this proteome data could be used as a research/reference resource by many sorghum and grain scientists worldwide. |
 Researcherดร. ฉัตรสุดา ศักดาเพชรศิริ, อาจารย์ที่ทำงาน:ภาควิชาโรคพืช คณะเกษตร กำแพงแสน สาขาที่สนใจ:Plant microbiome, Bioderagdation of xenobiotic substance, Bacterial Identification, Plant pathogenic bacteria, Bioformulation of biocontrol agents Resume |
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