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 หัวเรื่อง:การหมักโกโก้ II : การศึกษาอิทธิพลเอนไซม์เพคตินต่อการหมักโกโก้ธรรมชาติ ผู้เขียน: นางอรพิน ภูมิภมร, รองศาสตราจารย์ ,  Jantana Jinda สื่อสิ่งพิมพ์:pdf AbstractThe effects of enzyme pectinase on natural cocoa fermentation were studied by soaking cocoa beans in 200 ppm pectinase enzyme solution for a period of 0, 1, 3, 6 hour. The fermentation quality of fermented cocoa beans with added pectinase were shown to be more promising than those without enzyme batch . The amount of full fermented beans (4/4F) were increased concomitantly with the increasing of enzyme soaking period. Full fermented beans were found at 79 and 86% of fermented beans in enzyme – soaking batches of 3 and 6 hours respectively. No slaty and mold beans were observed in these batches. This indicated that the quality of fermented beans was equivalent to standard grade 1. Additionally, it was found that the pH levels of full fermented beans were 5.49 and 5.92 which were acceptable in the cocoa market. Pectinase –producing microorganism therefore, would have an important role in cocoa fermentation processes. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Sol-Gel of Rice Husk Ash: Entrapment of Alkaliphilic Lipase from Pseudomonas sp. KLB1) ผู้เขียน:Jantana Jinda, นางอรพิน ภูมิภมร, รองศาสตราจารย์, ดร.วิรัตน์ วาณิชย์ศรีรัตนา, รองศาสตราจารย์ , ดร.อรุณี อิงคากุล, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractEntrapment in sol-gel from rice husk ash was established by immobilization of alkali lipase produced by Pseudomonas sp. KLB1 lipase. The enzyme was strongly immobilized by entrapment in neutral sol- gel of rice husk ash. It was optimally active at 60?C and pH 9. Its activity was constant in the medium pH 9 under temperature 70?C for 1 hr. The immobilized lipase was activated by Ca2+, K+, Na+, EDTA and ascorbic acid but inhibited by Fe2+, Mn2+, Co2+, and KI. Both Ca2+,and Na+ were mainly found in rice husk ash and xerogel. The effective concentration of CaCl2 and NaCl on the enzyme activation were 60 mM and 20mM, respectively. According to reusability, it was applied into the hydrolysis of p-nitrophenyl palmitate by 12 times which lost 50% activity. The Km, and Vmax of p-NPP hydrolysis catalyzed by the entrapped enzyme were 7.43 ? 10–2 mM, and 1.94 ? 10-5 mM s-1, respectively, whilst by the free enzyme were 21.69 mM, and 3.90 ? 10-3 mM s-1, respectively. |
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หัวเรื่อง:การหมักโกโก้ : ศึกษาการเปล่ยนแปลงทางจุลนทรีย์ ฟิสิกส์ และเคมีในระหว่างการหมักโกโก้ ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์, Piyanuch Naka, Umpol Julsawad สื่อสิ่งพิมพ์:pdf AbstractStudies on environmental change during cocoa fermentation were carried out in baskets and wooden boxes, storing cocoa pods for 4 and 7 days after harvesting. During fermentation materials were sampled and ana?lysed at intervals. Beans fermented in boxes shiftly better cut test value than those kept in baskets. Fully fermented (F) bean of 54-6796 , and 28-4596 of 3/4 fermented beans were found in boxes, 50-5696 of fully fermented beans, 43?49% of 3/4 fermented beans in baskets. All fermented batches showed slaty less than 3%. The proximate analysis showed that fat content in unfermented and fermented beans were similar, at 5596, Ash and fiber in fermented beans was slightly less than in unfermented beans. The protein content decreased 1?296 by the end of the fermentation. The temperature during fermentation reached 45?C, after storage of cocoa pods for 4 days and 7 days, in 48 and 24 hours respectively. Mesophilic and thermophilic yeast and bacteria were also found. The pH of the fermented cocoa both in baskets and boxes were at 4.7-6.7 |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Biotreatment of High Fat and Oil Wastewater by Lipase Producing Microorganisms) ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์, อัจฉรา คอประเสริฐศักดิ์, Suptawee Funthong สื่อสิ่งพิมพ์:pdf AbstractStudies on biotreatments of high fat and oil wastewater by selected lipase producing bacteria were carried out in two experiments with two groups of bacteria, single culture and mixed culture. In the first experiment, wastewater of bakery industry was treated with 4 bacteria isolates. Results showed that grease in the form of fat and oil and COD decreased remarkably with treatments. With single culture, the removal of fat and oil and COD were 73%-88% and 81%-99% during 7 days treatment. Isolates KUL8 and KUL39 showed better activities. However, better treatments were found in experiment treated with single culture than using mixed culture. In the second experiment, wastewater of palm oil and bakery industries were treated with 6 isolates within 48 hours. All 6 isolates showed better degradation in palm oil wastes than bakery wastes. However, KUL8 and KUL39 still showed better degradation activities. Both isolates can removed fat and oil by 87.7% and 80.6% in palm oil wastewater and 70% and 64% in bakery wastes respectively. The decreasing of COD was found to be 90%-96%. When mixed culture of KUL8, KUL39 and KLB1 were applied for both kinds of wastewater. It was found that better results were observed in palm oil treated with single culture KUL8, KUL39, and KUB1 while mixed culture KUL8 mixed with KLB1 was suitable for use in bakery waste. The three isolates were identified as Acinetobacter sp. (KUL8), Bacillus sp. (KUL39), and Pseudomonas sp. (KLB1). They were all mesophilic strains. Besides, it was found that all three strains also produced amylase and protease which stimulate better waste treatments. |
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หัวเรื่อง:การหมักโกโก้ I : การจำแนกและศึกษาเมตาบอไลต์ของจุลินทรีย์จากการหมักโก้โก้ธรรมชาติ ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์, antana Jinda สื่อสิ่งพิมพ์:pdf Abstract840 isolated microorganisms during cocoa fermentation were studied. Sixhundred and thirty isolated bacteria were found in 4 families as Lactobacillaceae, Micro-coccaceae, Bacillaceae, Aectobactereae and in 5 genera of Lactobacillus sp., Micrococcus sp.Bacillus sp., Acetobacter sp. and Gluconobacter sp. Other 210 isolated yeasts were in family of Sporobolomycetaceae, Saccharomycetaceae and in genus of Candida sp., Kluyveromyces sp., and Saccharomyces sp. Two hundred and ten isolates each of yeast, bacteria and acetic acid bacteria were investigated for their efficiencies in alcohol (yeast), enzyme pectinase (yeast and bacteria) and acetic acid (acitic acid bacteria) formation. Yeasts produced ethyl alcohol in the amount of 0-10 mg/100 ml. Pectinase-producing yeasts and bacteria produced enzyme pectinase showed pectin degradation clear zone 0-9 mm. and 0-6 mm., respectively. Acetic acid bacteria produced acetic 0-0.4 gm/100 ml. According to the results of metabolites produced, eleven isolated microorganisms were chosen for further cocoa fermentation studied. The selected alcohol-producing yeasts were identified as Saccharomyces cerevisiae (KU-Y77), Candida utilis (KU-Y79), Candida krusei (KU-Y125); pectinase-producing yeasts and bacteria were Saccharomyces chevalier (KU-Y150) and Bacillus sp. (KU-B190), respectively; acetic bacteria were Acetobacter ranceus (KU-A40), Gluconobacter oxydans (KU-A45), Acetobacter acetic (KU-A72; KU-A187), Acetobacter pasteuriences (KU-A101) and Acetobacter persoxydans (KU-A132) |
หัวเรื่อง:การหมักโกโก้ IV: การประเมินคุณภาพทางเคมีและประสาทสัมผัสของโกโก้หมักด้วยกล้าเชื้อผสม ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์, Jantana Jinda, บุญให้ แหลมเพชร, Piyanut Naka สื่อสิ่งพิมพ์:pdf Abstract The quality of mix-culture fermented cocoa beans ere evaluated by sensory taste which related to some chemical substances. The fermented cocoa beans were received from 4 farm-trials in 4 days fermentation batches with full fermented beans of 79-87%, thus indicated in FAO grade 1 standard. Sensory taste of mix-culture fermented cocoa beans, evaluated by 20 trained panelists, indicated more preferable than the natural fermented one with preference score 6.0 – 7.5. The bitter astringent and acidity were reduced which related to the reduction of threobromine and glucose contents. The amount of threobromine and glucose were found to be 13.23-16.76 and 1.05-1.87 mg/g, respectively. The pH was 5.18-5.34 which was the pH range of accepted cocoa group. The organic acids that concerted to cocoa-flavor were acetic, lactic, citric and oxalic were found in the range of 0.60-2.89, 1.51-3.77, 2.83-3.34 and 0.49-0.56 mg/g respectively. According to proximate analysis, the protein and fat ( cocoa butter ) were in amount of 9.96-11.96% and 53.41-58.00% respectively with 61.65-67.75% saturated and 32.25-38.35% unsaturated fatty acid. As the results of experiment showed that the preference of mix-culture fermented cocoa beans were also related to some chemical substances in fermented cocoa which indicated to good quality cocoa beans. Thus, the improvement of cocoa fermentation with mix-culture can reduce fermentation time and also show better quality than the natural fermented one by without any changes in cocoa butter quality which was another good economic products. |
หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : Production of Glucoamylases by A Rotting Cassava Tuber Fungus on Solid Culture) ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractAspergillus niger H-9 is a fungal strain isolated from rotting cassava tuber in Thailand. In the present study, the strain was found to produce better glucoamylase on solid culture for 3-4 days. (ricebran and soybean meal in the ratio of 9:1). The moisture content for cultivation was found to be 3:1 of solid-liquid ratio. The suitable initial pH and temperature were 5-6 and 30 C. The highest enzyme yield was found to be 1,080 units. Optinum pH of the enzyme for starch hydrolysis was at pH 5.5. Such enzyme shows pH stability between 4-8, temperature activity at 60-70C and temperature stability between 30-70C. |
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หัวเรื่อง:ไม่มีชื่อไทย (ชื่ออังกฤษ : The Optimum use of Salinity, Nitrate and Pond Depth for b-Carotene Production of Dunaliella salina) ผู้เขียน:นางอรพิน ภูมิภมร, รองศาสตราจารย์, นายอุดม สิทธิภู่ประเสริฐ, อาจารย์, Naiyana Boontaveeyuwat, ดร.จันทนา ไพรบูรณ์, ผู้ช่วยศาสตราจารย์ สื่อสิ่งพิมพ์:pdf AbstractDunaliella salina, halotorelant green algae was collected from the East Coast of Thailand. It has a massive accumulation of b-carotene when grown under defined growth conditions such as high light intensity, high salt concentration and nitrate deficiency. The present study investigated the optimization of salinity, nitrate and pond depth for cell growth and b-carotene production of the alga in question. Cultivation was done in three stages. These were indoor cell growth cultivation, outdoor cell growth cultivation and outdoor b-carotene production. The optimum salinity for cell growth of indoor and outdoor cultivation (5 l) was 9% NaCl, which has specific growth rate (m) of 0.579 (d-1) and 0.981 (d-1). The optimum salinity for outdoors b-carotene production (5 l) when use 40% inoculum (2.16 ? 106 cell ml-1) was 12% NaCl. This produced b-carotene content of 51.73 mg ml-1. In addition, the concentrations of medium used were 100%, 75% and 50% to decrease nitrate concentration in the starter ponds for increasing b-carotene production in the next step. The results showed that 50% medium gave minimum nitrate concentration of 4.5 mg l-1. For the effect of pond depth, the light expose of the alga were used 9, 11, 13 and 18 cm the ratios of carotenoid to chlorophyll were 7.48, 6.25, 5.54 and 3.35, respectively. Therefore the suitable pond depth for b-carotene production from D. salina was 9 to 11 cm. |